■DestruxinA(DA)是从昆虫病原真菌金叶绿变菌中分离出的一种霉菌毒素,已显示出对各种昆虫物种的抑制活性。然而,对昆虫靶位点的抑制机制尚不清楚。
■在这项研究中,家蚕体内组织器官形态变化与DA的量效关系,家蚕,通过组织病理学方法进行研究,以确定对DA有反应的目标位点。
■结果表明,各个组织和器官的反应随DA剂量和治疗时间而变化。在低剂量下(即,0.01μg/g),血细胞对DA最敏感,处理后6h形态变化明显。然而,肌肉细胞,脂肪的身体,Malpighian小管没有改变.在较高剂量下(即,>0.1μg/g),在肌肉细胞中观察到形态变化,脂肪的身体,和治疗后24小时的Malpighian小管。结果表明,DA可以通过破坏宿主细胞如血细胞而成为免疫抑制剂,高剂量可能会影响其他生理过程,包括肌肉功能,新陈代谢,和排泄。当前研究中提供的信息将促进霉菌农药和新型免疫抑制剂的开发。
UNASSIGNED: Destruxin A (DA) is a mycotoxin isolated from the entomopathogenic fungus Metarhizium anisopliae which has demonstrated inhibitory activity against various insect species. However, the mechanism of inhibition on target sites in insects remains unknown.
UNASSIGNED: In this research, the dose-response relationship between DA and morphological changes in body tissues and organs of domestic silkworm, Bombyx mori, were investigated by histopathological methods to identify the target sites that responded to DA.
UNASSIGNED: The results showed that responses of individual tissues and organs varied with DA dosage and treatment time. At low doses (i.e., 0.01μg/g), the hemocytes were the most sensitive to DA with morphological changes apparent at 6 h after treatment. However, the muscle cells, fat body, and Malpighian tubules were unaltered. At higher doses (i.e., > 0.1μg/g), morphological changes were observed in muscle cells, fat body, and Malpighian tubules at 24 h after treatment. The results indicated that DA can be an immunosuppressant by damaging host cells like hemocytes, and at higher doses may potentially impact other physiological processes, including muscle function, metabolism, and excretion. The information presented in the current study will facilitate development of mycopesticides and novel immunosuppressants.