目的:COVID-19大流行导致许多公共设施中使用紫外线C(UVC)灭菌器的迅速传播。考虑到长期暴露于紫外线的有害影响,制造安全的护肤品是重要的对策。继续我们最近对水溶性草药提取物的研究,本研究旨在从脂溶性草药提取物中寻找抗UVC成分。
方法:将人真皮成纤维细胞和黑素瘤细胞暴露于UVC(1.193W/m2)3分钟。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物测定法测定活细胞数。使用细胞分选仪进行细胞周期分析。UVC保护活性通过选择性指数(SI)量化,即,未辐照细胞的50%细胞毒性浓度与使UVC处理的细胞活力恢复50%的浓度之比。
结果:只有柠檬草提取物,在12种脂溶性草药提取物中,显示出显着的抗UVC活性,与木质化材料和单宁相当,但超过N-乙酰-L-半胱氨酸和白藜芦醇。柠檬草提取物具有高度细胞毒性,产生亚G1细胞群。在培养基中长时间孵育期间,柠檬草提取物的抗UVC活性,抗坏血酸钠和香草酸的半衰期约<0.7、5.4-21.6和27.8-87.0h,分别。
结论:从柠檬草提取物中去除细胞毒性成分对于产生持久的UVC保护作用至关重要。
OBJECTIVE: The COVID-19 pandemic led to the rapid spread of the use of ultraviolet C (UVC) sterilizers in many public facilities. Considering the harmful effects of prolonged exposure to UVC, manufacturing of safe skin care products is an important countermeasure. In continuation of our recent study of water-soluble herbal extracts, the present study aimed at searching for anti-UVC components from fat-soluble herbal extracts.
METHODS: Human dermal fibroblast and melanoma cells were exposed to UVC (1.193 W/m2) for 3 min. Viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell-cycle analysis was performed using a cell sorter. UVC-protective activity was quantified by the selective index (SI), i.e., the ratio of the 50% cytotoxic concentration for unirradiated cells to the concentration that restored viability of UVC-treated cells by 50%.
RESULTS: Only lemongrass extract, among 12 fat-soluble herbal extracts, showed significant anti-UVC activity, comparable to that of lignified materials and tannins, but exceeding that of N-acetyl-L-cysteine and resveratrol. Lemongrass extract was highly cytotoxic, producing a subG1 cell population. During prolonged incubation in culture medium, the anti-UVC activity of lemongrass extract, sodium ascorbate and vanillic acid declined with an approximate half-life of <0.7, 5.4-21.6, and 27.8-87.0 h, respectively.
CONCLUSIONS: Removal of cytotoxic principle(s) from lemongrass extract is crucial to producing long-lasting UVC-protective effects.