■葡萄糖摄取可被认为是癌细胞生长和代谢的限速步骤。对GLUT1的研究表明,GLUT1参与健康和病理环境中的细胞存活和增殖。GLUT1表达被认为是发展局部侵略性的关键因素之一,肿瘤侵袭性,和转移,特别是在恶性肿瘤中。谷蛋白1在牙源性囊肿和肿瘤中的作用仍不确定。
■这项研究的目的是评估Glut1在牙质囊肿中的表达,牙源性角化囊肿,和成釉细胞瘤.
■该研究在GSL牙科学院进行。研究设计为再前瞻性免疫组织化学研究。
■福尔马林固定,组织学确诊病例的石蜡包埋块(n=50),10例牙源性角化囊肿,牙质囊肿,固体成釉细胞瘤,成釉细胞瘤单性,和每个牙齿卵泡。棕色染色被认为是GLUT1的阳性染色。通过计数标记细胞的数量进行定量分析,并通过分配免疫染色强度评分进行半定量分析。
■卡方检验用于比较各组之间的差异。P值≤0.05被认为具有统计学意义。
■牙源性角化囊肿和单囊性成釉细胞瘤显示≥50%的标记细胞具有强染色强度。牙源性角化囊肿和实体成釉细胞瘤在细胞质和膜中显示出染色的亚细胞定位。牙囊囊肿表现为合并核,细胞质,和染色的膜亚细胞定位。
■成釉细胞瘤的发展,牙源性角化囊肿,和牙质囊肿似乎受GLUT-1的影响。其表达的变化可能有助于解释这些病变的生物学活性的某些差异。
UNASSIGNED: Glucose uptake may be considered the rate-limiting step for the growth and metabolism of the cancer cell. Studies on GLUT1 have shown that GLUT1 is involved in cell survival and proliferation in both healthy and pathological circumstances. GLUT1 expression is regarded as one of the crucial elements in the development of local aggressiveness, tumour invasiveness, and metastasis, particularly in malignant tumours. The role of glut1 in odontogenic cysts and tumours has remained uncertain.
UNASSIGNED: The aim of the study is to assess the expression of Glut1 in dentigerous cysts, odontogenic keratocysts, and ameloblastoma.
UNASSIGNED: The study was conducted in GSL Dental College. The study design was a resprospective immunohistochemical study.
UNASSIGNED: Formalin-fixed, paraffin-embedded blocks of histologically confirmed cases (n = 50), 10 cases of odontogenic keratocysts, dentigerous cysts, ameloblastomas solid, ameloblastomas unicystic, and dental follicles each. Brown colour staining was considered as positive staining for GLUT1. Quantitative analysis was performed by counting the number of labelled cells, and semi-quantitative analysis was conducted by assigning immunostaining intensity scores.
UNASSIGNED: Chi-square test was used to compare differences between the groups. A P value of ≤0.05 was considered as statistically significant.
UNASSIGNED: Odontogenic keratocysts and unicystic ameloblastoma showed ≥50% of label cells with strong intensity of staining. Odontogenic keratocysts and solid ameloblastoma showed sub-cellular localisation of staining in the cytoplasm and membrane. Dentigerous cysts exhibited combined nucleus, cytoplasm, and membrane sub-cellular localisation of staining.
UNASSIGNED: The development of ameloblastomas, odontogenic keratocysts, and dentigerous cysts appears to be influenced by GLUT-1. Variation in its expression may aid in explanation of some of the differences in biological activity of these lesions.