在这项研究中,由氯化胆碱制备的低共熔溶剂(DES),乳酸,和四种多元醇之一(乙二醇,甘油,木糖醇,和山梨糖醇)进行了比较,并评估了它们从龙虾壳中提取甲壳质的有效性。我们的结果表明,随着多元醇中羟基数量的增加,DES内的氢键网络变得更加密集。然而,这导致粘度相应增加,这影响了甲壳素的提取效率。在所有准备好的DES中,氯化胆碱-乳酸/甘油(CCLaGly)表现出优异的提取能力,从龙虾壳中提取纯甲壳素。纯度,结晶度使用CCLaGlyDES提取的几丁质的分子量与化学分离的几丁质的分子量相当,纯度达到94.76±0.33%,结晶度为78.78%,分子量为655kDa。此外,DES提取的几丁质的理化性质使用傅里叶变换红外光谱进行了表征,X射线衍射,热重分析,和扫描电子显微镜。本研究对多元醇对从龙虾壳中提取几丁质的影响进行了比较分析,从而为在可持续生物材料生产中利用各种甲壳类贝壳开辟了一条有希望的途径。
In this study, deep eutectic solvent (DES) prepared from choline chloride, lactic acid, and one of the four polyols (ethylene glycol, glycerol, xylitol, and sorbitol) were compared and assessed for their effectiveness in extracting chitin from lobster shells. Our results revealed that as the number of hydroxyl groups in polyols increased, the hydrogen bond network within the DESs became denser. However, this led to a corresponding increase in viscosity, which impacted the efficiency of chitin extraction. Among all prepared DESs, choline chloride-lactic acid/glycerol (CCLaGly) exhibited superior extractive ability, resulting in the extraction of pure chitin from lobster shells. The purity, crystallinity, and molecular weight of the extracted chitin using CCLaGly DES were comparable to those of chemically-isolated chitin, with purity reaching 94.76 ± 0.33 %, crystallinity at 78.78 %, and a molecular weight of 655 kDa. Additionally, the physicochemical properties of the DES-extracted chitins were characterized using Fourier-transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, and scanning electron microscopy. This study conducted a comparative analysis of polyol effects on chitin extraction from lobster shells, thereby opening a promising avenue for the utilization of various crustacean shells in sustainable biomaterial production.