Breeding herds in the US are trending toward eradication of Mycoplasma hyopneumoniae (M. hyopneumoniae) due to the positive impact on welfare and downstream production. In an eradication program, \"Day 0″ is the time point when the last replacement gilts to enter the herd were exposed to M. hyopneumoniae and marks the beginning of a herd closure. However, no specific diagnostic protocols are available for confirmation of successful exposure to define Day 0. Therefore, the objective of this study was to develop diagnostic guidelines, including sample collection approaches, for two common gilt exposure methods to confirm an entire population has been infected with M. hyopneumoniae following purposeful exposure. Forty gilts, age 21-56 days, were ear-tagged for longitudinal sample collection at five commercial gilt developer units (GDUs) and were exposed to M. hyopneumoniae by natural contact or aerosolization. Study gilts originated from sources known to be negative to major swine pathogens, including M. hyopneumoniae, and were sampled prior to exposure to confirm negative status, then every two weeks. Blood samples were collected for antibody detection, while laryngeal and deep tracheal secretions and pen based oral fluids were collected for PCR, and sampling continued until at least 85% of samples were positive by PCR. Detection of M. hyopneumoniae varied greatly based on sample type. Oral fluids showed the lowest detection in groups of gilts detected positive by other sample types. Detection by PCR in deep tracheal secretions was higher than in laryngeal secretions. Seroconversion to and PCR detection of M. hyopneumoniae on oral fluids were delayed compared to PCR detection at the individual level. By two weeks post-exposure, at least 85% of study gilts in three GDUs exposed by aerosolization were PCR positive in deep tracheal secretions. Natural contact exposure resulted in 22.5% of study gilts becoming PCR positive by two weeks post-initial exposure, 61.5% at four weeks, and 100% at six weeks on deep tracheal secretions. Deep tracheal secretions required the lowest number of gilts to sample for the earliest detection compared to all other samples evaluated. As observed in one of the GDUs using aerosolization, demonstration of failure to expose gilts to M. hyopneumoniae allowed for early intervention in the exposure protocol and delay of Day 0, for accurate timing of the eradication protocol. Sampling guidelines proposed in this study can be used for verification of M. hyopneumoniae infection of gilts following exposure to determine Day 0 of herd closure.

Herein, we are critically examining the chain of events and discussing previously unrecognized factors that led to the \'perfect COVID-19 storm\' in northern Italy during the first epidemic wave in spring 2020. SARS-CoV-2 was circulating uncontrollably at least for five weeks before the adoption of containment measures, and the role of exponential growth in the spread of the virus, conveyed by a high R0, was likely underestimated. An understanding of this failure\'s causes and contexts will help us to control the strong second wave of the pandemic we are now facing in Europe, and to be better prepared for future outbreaks.

The challenges associated with climate change in local governments are growing daily. One such challenge is water security, an aspect that draws us to the subject matter of climate change adaptation. This article discusses findings about institutional complexities surrounding Day Zero, a concept associated with water taps running dry because of drought conditions as aggravated by climate change in the city of Cape Town, South Africa. The thrust on institutional complexities is deliberate, as this affects how crisis situations like Day Zero were handled. The data were generated mainly from the actor-actant-network theory, events study as well as document and discourse analysis methods. The actor-actant-network theory is used widely to trace how actors (humans) and actants (non-human phenomena) interact in space and time through their networks, following narratives like Day Zero, and act on climate-related matters. The analysis applied elements of grounded theory, resulting in categories and themes emerging for discussion. The article found that narratives surrounding Day Zero were embedded in both political and administrative dilemmas and red tape. Despite these challenges, the article concludes that Day Zero remains one of the landmark learning points for climate change adaptation and water security in Cape Town, South Africa, and in other cities across the world. The article recommends that Day Zero experiences continue to be embraced positively and documented further to enhance local government climate adaptation for water security currently and into the future as well.