本文提出并开发了一种用于测量污泥好氧造粒潜力的协议,旨在提供一种经济实惠且简单的替代方案,以促进好氧制粒技术的发展。在这个意义上,协议包括一组参数和考虑因素,这些参数和考虑因素相互作用以创建受控环境并刺激细胞群体聚集。所有这些都是在程序简单的背景下完成的,低成本,以及获得结果的速度。该协议本质上是一个三阶段的方法:准备基板,接种物的适应,和协议的实现。测量简单参数以评估造粒过程:SVI,沉降速度,和形态参数。该方案根据文献中先前建立的最佳范围和标准进行验证。为此,来自生活污水处理厂的活性污泥接种物被提交到协议中,在相对较短的时间(7d)内获得生物量的最佳响应(SVI5=13.90mLg-1,沉降速度=25,79mh-1,直径>0.2mm)。结果表明,该协议可以构成使用传统实验室设备进行评估和决策的工具,并且适用于不同的规模。
This paper proposes and develops a protocol for measuring the aerobic granulation potential of sludge, aiming to provide an affordable and simple alternative that can facilitate the development of aerobic granulation technology. In this sense, the protocol comprises a set of parameters and considerations that interact to create a controlled environment and stimulate cell population clustering. All of this is done in the context of procedural simplicity, low cost, and the speed at which results are obtained. The protocol is essentially a three-stage method: preparation of the substrate, adaptation of the inoculum, and implementation of the protocol. Simple parameters were measured to evaluate the granulation process: SVI, settling velocity, and morphological parameters. The protocol was validated according to optimal ranges and criteria previously established in the literature. For this purpose, an activated sludge inoculum from a domestic wastewater treatment plant was submitted to the protocol, obtaining an optimal response of the biomass (SVI5 =13.90 mL g-1, settling velocity= 25,79 m h-1, Diameter > 0.2 mm) in a relatively short time (7 d). The results show that this protocol can constitute a tool for evaluation and decision-making using traditional laboratory equipment and is applicable at different scales.