滚环扩增(RCA)是一种等温酶促扩增反应,用于大量产生具有预先设计的序列和功能的周期性长单链DNA/RNA。RCA技术不仅在构建功能性DNA组件方面显示出无与伦比的优势;RCA产品还可以作为高性能支架与外源部分相互作用或容纳外源部分,即,治疗性核酸,蛋白质,脂质,阳离子聚合物,和金属纳米颗粒(NPs),等。制造具有各种尺寸和形态的多功能和生物响应材料。RCA产品也是一种具有强分子间/分子内相互作用的高分子量聚阴离子,这为调节DNA纳米药物和散装材料的缩合状态提供了很好的机会。基于所有这些良好的特性,基于RCA的材料在生物医学领域显示出越来越多的应用潜力。在这次审查中,我们总结了基于RCA的折纸结构的最新发展,NPs,水凝胶,和金属化,制造方法,它们的生物医学应用,并仔细讨论了未来的前景。
Rolling circle amplification (RCA) is an isothermal enzymatic amplification reaction, which is used to massively produce periodic long-single-stranded DNA/RNA with predesigned sequences and functions. The RCA technique not only shows unparalleled advantages in constructing functional DNA assemblies; and the RCA products can also serve as high-performance scaffolds to interact with or accommodate foreign moieties, i.e., therapeutic nucleic acids, proteins, lipids, cationic polymers, and metal nanoparticles (NPs), etc. to fabricate multifunctional and bioresponsive materials with various sizes and morphologies. The RCA product is also a kind of high-molecular-weight polyanion with strong inter/intra-molecular interactions, which provides great opportunities for regulating the condensation states of DNA nanomedicines and bulk materials. Based on all these good properties, the RCA-based materials have shown more and more practical potentials in biomedical fields. In this review, we summarize the recent development of RCA-based origami structures, NPs, hydrogels, and metallization, for which the fabrication methods, their biomedical applications, and future prospects are carefully discussed.