背景:Murrah水牛,在亚洲农业中举足轻重,尽管进行了大量的育种工作,但在最大限度地提高牛奶产量方面仍面临挑战。认识到其经济重要性,这项研究调查mtDNAD-loop变异在Murrah水牛作为潜在指标的产奶变异性,尽管进行了大量育种工作,但仍要解决最大产量方面的挑战。
结果:分析50只水牛的mtDNAD环序列,我们将它们分类为低(第1组),中等(第2组),和基于产奶量的高ECM(第3组)组,30天期间数据的脂肪和蛋白质百分比。体细胞mtDNAD-loop分析揭示了不同的遗传变异,ECM组间差异显著。第2组显示更高的SNP患病率,第3组有更多的插入/缺失,第1组表现出最高的跃迁频率。值得注意的是,第714位出现一致的“C”缺失,发生在第1组和第3组中,占第2组的68%。在第93位的G-A变异对培养基ECM组是特异性的。TajimaD的负值表明每组有独特的变化,第1组的数字最高,并鉴定了与组2相关的特定SNP。D-loop区域中的这些SNP会影响mtDNA复制,影响动物线粒体含量。我们的结果为mtDNAD环多态性在Murrah水牛产奶性状中的作用提供了有价值的见解。
结论:我们的研究强调了Murrah水牛细胞能量效率的有价值标志物的潜力。探索不同的细胞质背景为基于mtDNA的选择策略开辟了道路,提高乳品产量,优化乳品行业的遗传性状。
BACKGROUND: The Murrah buffalo, pivotal in Asian agriculture, faces challenges in maximizing milk production despite significant breeding efforts. Recognizing its economic importance, this study investigates mtDNA D-loop variations in Murrah buffalo as potential indicators of milk production variability, addressing challenges in maximizing yield despite significant breeding efforts.
RESULTS: Analyzing mtDNA D-loop sequences from 50 buffaloes, we categorized them into Low (Group 1), Medium (Group 2), and High ECM (Group 3) groups based on milk yields, fat and protein percentage of a 30-day period data. Somatic cell mtDNA D-loop analysis revealed distinct genetic variations, with significant differences among ECM groups. Group 2 showed higher SNP prevalence, group 3 had more insertions/deletions, and Group 1 exhibited the highest transition frequency. Notably, a consistent \"C\" deletion at the 714th position occurred in Groups 1 and 3, prevalent in 68% of Group 2. A G-A variation at the 93rd position was specific to the medium ECM group. Negative Tajima D values indicated unique variations in each group, with Group 1 having the highest number, and a specific SNP linked to Group 2 was identified. These SNPs in the D-loop region could impact mtDNA replication, influencing mitochondrial content among animals. Our results provide valuable insights into the role of mtDNA D-loop polymorphisms in milk production traits in Murrah buffalo.
CONCLUSIONS: Our research highlights the potential for valuable markers of cellular energy efficiency in Murrah buffalo. Exploring diverse cytoplasmic backgrounds opens avenues for mtDNA-based selection strategies, enhancing milk production and optimizing genetic traits for the dairy industry.