腺苷酸与动物器官发育和肿瘤发生密切相关。Dally-like(Dlp),膜结合的磷脂酰肌醇蛋白聚糖,在果蝇的各种生物过程中起着关键作用。在这项研究中,我们观察到过量的Dlp会导致腿部畸形,特别是影响远端部分。因此,腿椎间盘收缩,经常表现出形态异常。此外,升高的Dlp水平诱导异位细胞死亡,没有明显的细胞增殖变化。此外,后室中的Dlp过表达显着改变了无翼(Wg)分布。我们观察到后房室内Wg分布明显扩大,伴随着前室的相应减少。似乎过量的Dlp引导Wg扩散至具有较高Dlp水平的细胞。此外,非远端(dll)基因,这对腿部造型至关重要,显著上调。值得注意的是,腊肠(dac)和同胸(hth)表达式,也是必不可少的腿部图案和发展,似乎只受到了可忽略的影响。基于这些发现,我们推测过量的Dlp可能导致果蝇远端腿区域的畸形,可能是通过它对WG分布的影响,dll表达和诱导细胞死亡。我们的研究促进了对果蝇腿发育中Dlp功能的理解。
Glypicans are closely associated with organ development and tumorigenesis in animals. Dally-like (Dlp), a membrane-bound glypican, plays pivotal roles in various biological processes in Drosophila. In this study, we observed that an excess of Dlp led to the malformation of legs, particularly affecting the distal part. Accordingly, the leg disc was shrunken and frequently exhibited aberrant morphology. In addition, elevated Dlp levels induced ectopic cell death with no apparent cell proliferation changes. Furthermore, Dlp overexpression in the posterior compartment significantly altered Wingless (Wg) distribution. We observed a marked expansion of Wg distribution within the posterior compartment, accompanied by a corresponding decrease in the anterior compartment. It appears that excess Dlp guides Wg to diffuse to cells with higher Dlp levels. In addition, the distal-less (
dll) gene, which is crucial for leg patterning, was up-regulated significantly. Notably, dachshund (dac) and homothorax (hth) expression, also essential for leg patterning and development, only appeared to be negligibly affected. Based on these findings, we speculate that excess Dlp may contribute to malformations of the distal leg region of Drosophila, possibly through its influence on Wg distribution,
dll expression and induced cell death. Our research advances the understanding of Dlp function in Drosophila leg development.