Bacterial extracellular vesicles (BEVs) are nano-size vesicles containing a cargo of bioactive molecules that can play key roles in microbe-microbe and microbe-host interactions. In tracking their biodistribution in vivo, BEVs can cross several physical host barriers including the intestinal epithelium, vascular endothelium, and blood-brain-barrier (BBB) to ultimately accumulate in tissues such as the liver, lungs, spleen, and the brain. This tissue-specific dissemination has been exploited for the delivery of biomolecules such as vaccines for mucosal delivery. Although numerous strategies for labeling and tracking BEVs have been described, most have constraints that impact on interpreting in vivo bioimaging patterns. Here, we describe a general method for labeling BEVs using lipophilic fluorescent membrane stains which can be adopted by non-expert users. We also describe how the procedure can be used to overcome potential limitations. Furthermore, we outline methods of quantitative ex vivo tissue imaging that can be used to evaluate BEV organ trafficking.

UNASSIGNED: As one of the pioneering pilot cities in China\'s extensive Diagnosis Related Groups (DRG) -based prepayment reform, Beijing is leading a comprehensive overhaul of the prepayment system, encompassing hospitals of varying affiliations and tiers. This systematic transformation is rooted in extensive patient group data, with the commencement of actual payments on March 15, 2022. This study aims to evaluate the effectiveness of DRG payment reform by examining how it affects the cost, volume, and utilization of care for patients with neurological disorders.
UNASSIGNED: Utilizing the exogenous shock resulting from the implementation of the DRG-based prepayment system, we adopted the Difference-in-Differences (DID) approach to discern changes in outcome variables among DRG payment cases, in comparison to control cases, both before and following the enactment of the DRG policy. The analytical dataset was derived from patients diagnosed with neurological disorders across all hospitals in Beijing that underwent the DRG-based prepayment reform. Strict data inclusion and exclusion criteria, including reasonableness tests, were applied, defining the pre-reform timeframe as March 15th through October 31st, 2021, and the post-reform timeframe as the corresponding period in 2022. The extensive dataset encompassed 53 hospitals and encompassed hundreds of thousands of cases.
UNASSIGNED: The implementation of DRG-based prepayment resulted in a substantial 12.6% decrease in total costs per case and a reduction of 0.96 days in length of stay. Additionally, the reform was correlated with significant reductions in overall in-hospital mortality and readmission rates. Surprisingly, the study unearthed unintended consequences, including a significant reduction in the proportion of inpatient cases classified as surgical patients and the Case Mix Index (CMI), indicating potential strategic adjustments by providers in response to the introduction of DRG payments.
UNASSIGNED: The DRG payment reform demonstrates substantial effects in restraining cost escalation and enhancing quality. Nevertheless, caution must be exercised to mitigate potential issues such as patient selection bias and upcoding.

The Covid pandemic changed the daily routines for millions of people. This was the case for those who were gainfully employed, especially for those who work as psychoanalysts and psychodynamic psychotherapists. At least for a good while, the practice of psychotherapy and psychoanalysis moved from the consulting room to the virtual world of the internet. The author explores the impact virtual therapy had on three different patients. One began a three time a week analysis during the pandemic. The duo met virtually for a year and a half before their first in person meeting. The other two patients had begun twice a week analyses a few years before the pandemic, met virtually for two years, until in person sessions restarted. The patients and the author describe their experiences.

BACKGROUND: The fragmentation of the medical insurance system is a major challenge to achieving health equity. In response to this problem, the Chinese government is pushing to establish the unified Urban and Rural Resident Basic Medical Insurance (URRBMI) system by integrating the New Rural Cooperative Medical Scheme and the Urban Resident Basic Medical Insurance. By the end of 2020, URRBMI had been implemented almost entirely across China. Has URRBMI integration promoted health equity for urban and rural residents?
OBJECTIVE: This study aims to examine the effect of URRBMI integration on the health level of residents and whether the integration can contribute to reducing health disparities and promoting health equity.
METHODS: We used the staggered difference-in-differences method based on the China Family Panel Studies survey from 2014 to 2018. Our study had a nationally representative sample of 27,408 individuals from 98 cities. We chose self-rated health as the measurement of health status. In order to more accurately discern whether the sample was covered by URRBMI, we obtained the exact integration time of URRBMI according to the official documents issued by local governments. Finally, we grouped the sample by urban and rural areas, regions, and household income to examine the impact of the integration on health equity.
RESULTS: We found that overall, the URRBMI integration has improved the health level of Chinese residents (B=0.066, 95% CI 0.014-0.123; P=.01). In terms of health equity, the results showed that first, the integration has improved the health level of rural residents (B=0.070, 95% CI 0.012-0.128; P=.02), residents in western China (B=0.159, 95% CI 0.064-0.255; P<.001), and lower-middle-income groups (B=0.113, 95% CI 0.004-0.222, P=.04), so the integration has played a certain role in narrowing the health gap between urban and rural areas, different regions, and different income levels. Through further mechanism analysis, we found that the URRBMI integration reduced health inequity in China by facilitating access to higher-rated hospitals and increasing reimbursement rates for medical expenses. However, the integration did not improve the health of the central region and low-income groups, and the lack of access to health care for low-income groups was not effectively reduced.
CONCLUSIONS: The role of URRBMI integration in promoting health equity among urban and rural residents was significant (P=.02), but in different regions and income groups, it was limited. Focusing on the rational allocation of medical resources between regions and increasing the policy tilt toward low-income groups could help improve the equity of health insurance integration.

This study investigates the practical effects of adopting the environmental protection tax (EPT) policy on corporate performance in China. The analysis uses the Difference in Differences (DID) approach based on a quasi-natural experiment scenario. The findings indicate there is a negative impact of implementing the EPT policy on the financial performance of corporations, and the conclusion remains unchanged despite exhaustive robustness testing. The negative impact can be partly attributed to corporate technology innovation inputs. Meanwhile, enterprise property rights, pollution, and technical levels also substantially influence the implementation effect of the legislation. However, implementing this policy has improved corporations\' environmental performance and established its efficacy in enhancing their sustainable capabilities. This study comprehensively explores the impact of environmental control legislation on business performance, spanning financial, environmental, and social dimensions. Corresponding findings offer valuable insights into how firms react to environmental legislation and adjust to the external environment. Meanwhile, it also provides an objective reference for the comprehensive green transformation.

This study investigates the impact of marriage on the body mass index (BMI) of individuals aged 18-45 in China. We used data from ten rounds of the China Health and Nutrition Survey spanning from 1989 to 2015, and applied Difference-in-Differences (DID) model to examine the impact of marriage on BMI. Our findings reveal that marriage has a significant positive effect on BMI, especially among males, with post-marriage elevation of male BMI continuing to increase over time. Moreover, marriage is associated with a 5.2% increase in the prevalence of overweight and a 2.5% rise in the incidence of obesity among males. While energy intake levels for Chinese women did not undergo significant changes after marriage, nor did their energy expenditure levels, men experienced a marked alteration in energy balance, characterized by an increase in caloric intake and a decrease in physical activity. Furthermore, our study confirms significant period differences in the effect of marriage on BMI, with post-marriage elevation of male BMI continuing to rise over time. In contrast, there was no clear trend for female BMI after marriage. Our research highlights the importance of promoting physical fitness and health management within families while fostering intimate relationships through marriage. Public health policies should consider the potential impact of marriage as an intervention window for addressing individuals\' weight management needs. Distinct post-marriage body management plans should be designed for both genders.

Dissociative identity disorder (DID), commonly known as multiple personality disorder (MPD), is a contentious mental health condition that typically arises as a result of traumatic events to help people avoid unpleasant memories. To completely comprehend the complexity and nuance of DID, this study investigates its symptomatology, diagnostic criteria, therapeutic modalities, and historical controversies. Patients with DID frequently have two or more distinct personality identities, each with its memories, characteristics, and attributes. Ten personality disorders are listed in the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, Text Revision (DSM-5-TR), but DID, formerly known as MPD, is not one of those personality disorders. Nevertheless, myths and misunderstandings cloud our knowledge of the disease, and some critics attribute the condition\'s emergence to therapy rather than trauma. This study emphasizes the possibilities for recovery and fulfilling life for persons affected by DID by attempting to provide a comprehensive understanding of DID, debunk myths and misconceptions, and throw light on effective therapy methods. It accomplishes this by carefully examining the body of literature and existing studies. The DID study used a systematic strategy to obtain a thorough grasp of the causes, diagnosis, symptoms, and therapies of the disorder. It employed precise keywords and Boolean operators across four databases, prioritized current peer-reviewed English-language publications, and enforced strict exclusion standards. While admitting potential biases and limits in the databases used, the research intended to maintain methodological transparency and robustness, helping to provide an accurate and up-to-date picture of DID.

Intelligent Phytoprotection is an important direction for the modern development of plant protection related disciplines, and its essence is the innovative application of new generation information technology industry, high-end equipment manufacturing industry, and digital industry related technologies in the traditional plant protection field. This article first identifies 224 International Patent Classification (IPC) Main groups in the field of intelligent phytoprotection technology based on the International Patent Classification System. And then combines with China\'s industrial policy practice, we explore the impact of industrial policy on the application number of invention patents in the field of intelligent phytoprotection technology using the Difference-in-difference (DID) method and the Synthetic DID method. The study results showed that the implementation of industrial policy can significantly promote the patent application activities in the intelligent phytoprotection treatment group, with an average increase of 517 invention patent applications compared to the control group that is not affected by the policy. The research conclusion of this article suggests that for countries and regions, industrial policies are an important tool for promoting the innovation and development of intelligent phytoprotection related technologies.

Drawing on the textual contents of the Chinese local government work reports, this paper focuses on the impact of leading officials\' audits of natural-resource assets on the local environmental attention allocation. The study has found that during the pilot period of audits, compared with the non-pilot cities, the local government in the pilot cities has concentrated more on the ecological environment field and has placed greater emphasis on the actual environmental governance. The results of mechanism analysis show that differences in the personal and take-office characteristics of local officials, may lead to different promotion pressure and performance evaluation modes, and then have a different impact on local environmental attention. The test results of consequence analysis show that the environmental attention in the pilot cities can be further transformed into environmental administrative and financial governance behavior. The paper discusses the policy effect and internal mechanism of leading officials\' audits of natural-resource assets from the perspective of local governments\' environmental attention, providing empirical evidence for China to carry out the top-level design of environmental policy based on the idea of optimizing officials\' performance evaluation mechanism.

Within the context of promotion tournaments among local governments, the management of economic growth goals plays a crucial role in China\'s economic development. Despite China\'s rise as the second-largest economy globally, it has also emerged as the largest emitter of carbon emissions. Since the implementation of the 12th Five-Year Plan in 2011, the Chinese central government has made carbon intensity targets mandatory indicators for national economic development. This has prompted local governments to pursue low-carbon growth and adjust their economic growth targets (EGT) to comply with carbon intensity constraints. In this study, a sample of 282 prefecture-level cities in China is used to empirically examine the impact of carbon intensity constraints on total factor carbon emission efficiency (TCE) using the intensity difference-in-differences (DID) framework. The study also emphasizes the role of the transmission channel for economic growth pressure (EGP). The findings of the study reveal several key results. Firstly, the implementation of carbon intensity constraints leads to an average increase of 8.24% in total factor carbon emission efficiency (TCE), which is supported by robustness tests, parallel trend analysis, and placebo tests. Secondly, these constraints result in an average decrease of 0.1828 in local governments\' economic growth targets (EGT) and a reduction of 0.1269 in economic growth pressure (EGP). Thirdly, cities with a higher proportion of secondary industry experience a more significant mitigation effect, although the promotion of provincial EGT hinders this effect. Fourthly, synergistic policies can effectively promote low-carbon development, and government expenditure on technology and marketization can facilitate a positive relationship between carbon intensity constraints and TCE. Lastly, the effects of carbon intensity constraints vary across the east, middle, and west regions, suggesting the presence of heterogeneity. The article proposes a shift in the assessment of lower governments by superior governments, from growth assessment to low-carbon growth assessment.