本研究调查了大麻(CannabissativaL.)叶片的植物化学成分和生物活性,来自索非亚品种的花\'甲醇提取物,以及在不同光照条件下种植的豆芽(自然光,黑暗,蓝色,和白色LED灯持续5、7和9天)。使用HPLC进行的植物化学分析确定了豆芽提取物中的四种关键多酚化合物:绿原,咖啡因,和没食子酸,还有杨梅素,绿原酸占主导地位。相比之下,叶子和花的提取物含有山黄A和B以及绿原,p-coumaric,和阿魏酸,具有显著的异绿原酸的存在。抗氧化能力,用FRAP方法评估,与花和豆芽相比,叶子中的抗氧化能力更高,在蓝色和白色LED灯下生长的豆芽表现出最高的活性。在人结肠癌细胞系(HT29、HCT116、DLD-1)和正常结肠上皮细胞(CCD841CoN)上评价细胞毒性活性。结果表明,对癌细胞系具有显著和选择性的细胞毒性,叶子比花表现出更明显的效果,和豆芽只有适度的活动。所有样品均显示出体外抗炎作用。最后,豆芽,叶子,和花的索非亚大麻可能被认为是有前途的产品在未来的化学预防。
This study investigates the phytochemical composition and biological activities of hemp (Cannabis sativa L.) leaves, flowers\' methanolic extracts from the Sofia variety, and its sprouts cultivated under different light conditions (natural light, darkness, blue, and white LED light for 5, 7, and 9 days). Phytochemical analysis using HPLC identified four key polyphenolic compounds in sprouts\' extracts: chlorogenic, caffeic, and gallic acids, and myricetin, with a predomination of the chlorogenic acid. In contrast, leaves and flowers\' extracts contained cannflavins A and B and chlorogenic, p-coumaric, and ferulic acids, with a significant presence of isochlorogenic acid. Antioxidant capacity, assessed by FRAP method, revealed higher antioxidant potential in leaves compared to flowers and sprouts, with sprouts grown under blue and white LED lights exhibiting the highest activity.
Cytotoxic activity was evaluated on human colon cancer cell lines (HT29, HCT116, DLD-1) and normal colon epithelial cells (CCD 841 CoN). Results demonstrated significant and selective cytotoxicity against cancer cell lines, with leaves showing more pronounced effects than flowers, and sprouts only moderate activity. All samples revealed an anti-inflammatory effect in vitro. To conclude, sprouts, leaves, and flowers of the Sofia hemp may be considered promising products for chemoprevention in the future.