Mitosis is a crucial stage in the cell cycle, controlled by a vast network of regulators responding to multiple internal and external factors. The fission yeast Schizosaccharomyces pombe demonstrates catastrophic mitotic phenotypes due to mutations or drug treatments. One of the factors provoking catastrophic mitosis is a disturbed lipid metabolism, resulting from, for example, mutations in the acetyl-CoA/biotin carboxylase (cut6), fatty acid synthase (fas2, also known as lsd1) or transcriptional regulator of lipid metabolism (cbf11) genes, as well as treatment with inhibitors of fatty acid synthesis. It has been previously shown that mitotic fidelity in lipid metabolism mutants can be partially rescued by ammonium chloride supplementation. In this study, we demonstrate that mitotic fidelity can be improved by multiple nitrogen sources. Moreover, this improvement is not limited to lipid metabolism disturbances but also applies to a number of unrelated mitotic mutants. Interestingly, the partial rescue is not achieved by restoring the lipid metabolism state, but rather indirectly. Our results highlight a novel role for nitrogen availability in mitotic fidelity.

Butterfly wings display a diversity of cell types, including large polyploid scale cells, yet the molecular basis of such diversity is poorly understood. To explore scale cell diversity at a transcriptomic level, we employ single-cell RNA sequencing of ∼5,200 large cells (>6 μm) from 22.5- to 25-h male pupal forewings of the butterfly Bicyclus anynana. Using unsupervised clustering, followed by in situ hybridization, immunofluorescence, and CRISPR-Cas9 editing of candidate genes, we annotate various cell types on the wing. We identify genes marking non-innervated scale cells, pheromone-producing glandular cells, and innervated sensory cell types. We show that senseless, a zinc-finger transcription factor, and HR38, a hormone receptor, determine the identity, size, and color of different scale cell types and are important regulators of scale cell differentiation. This dataset and the identification of various wing cell-type markers provide a foundation to compare and explore scale cell-type diversification across arthropod species.

Cortex glia in Drosophila central nervous system form a niche around neural cells for necessary signals to establish cross talk with their surroundings. These cells grow and expand their thin processes around neural cell bodies. Although essential for the development and function of the nervous system, how these cells make extensive and intricate connected networks remains largely unknown. In this study, we show that Cut, a homeodomain transcription factor, directly regulates the fate of the cortex glia, impacting neural stem cell (NSC) homeostasis. Focusing on the thoracic ventral nerve cord, we found that Cut is required for the normal growth and development of cortex glia and timely increase in DNA content through endocycle to later divide via acytokinetic mitosis. Knockdown of Cut in cortex glia significantly reduces the growth of cellular processes, the network around NSCs, and their progeny\'s cell bodies. Conversely, overexpression of Cut induces overall growth of the main processes at the expense of side ones. Whereas the Cut knockdown slows down the timely increase of DNA, the Cut overexpression results in a significant increase in nuclear size and volume and a 3-fold increase in DNA content of cortex glia. Further, we note that constitutively high Cut also interfered with nuclei separation during acytokinetic mitosis. Since the cortex glia form syncytial networks around neural cells, the finding identifies Cut as a novel regulator of glial growth and variant cell cycles to support a functional nervous system.

BACKGROUND: Athletes with a history of concussion are at a greater risk for lower extremity musculoskeletal injury. Female athletes may be at an even greater risk than male athletes. Previous researchers on postconcussion landing biomechanics have focused on the lower extremities, but the trunk plays a crucial role as an injury risk factor.
OBJECTIVE: To compare lower extremity and trunk biomechanics during jump-landing and cutting maneuvers between female athletes with and those without a concussion history.
METHODS: Cross-sectional study.
METHODS: Biomechanics laboratory.
METHODS: A total of 26 athletes (mean ± SD age = 19.0 ± 1.3 years, height = 1.68 ± 0.07 m, mass = 64.02 ± 6.76 kg, body mass index = 22.58 ± 1.97 kg/m2; median [interquartile range] time since most recent concussion = 37.5 months [25.0 months, 65.8 months]) with a concussion history and 38 athletes (age = 19.0 ± 1.1 years, height = 1.71 ± 0.08 m, mass = 64.72 ± 9.45 kg, body mass index = 22.14 ± 1.80 kg/m2) without a concussion history.
METHODS: Peak kinetics (vertical ground reaction force, vertical loading rate, external knee-abduction moment, and external knee-flexion moment) and kinematics (trunk-flexion angle, trunk lateral-bending angle, ankle-dorsiflexion angle, knee-flexion angle, knee-abduction angle, and hip-flexion angle) were obtained during the eccentric portion of jump-landing and cutting tasks. Separate 2 (group) × 2 (limb) between- and within-factors analyses of covariance were used to compare outcomes between groups. We covaried for time since the most recent concussion and the limb that had a history of musculoskeletal injury.
RESULTS: Athletes with a concussion history displayed a greater peak knee-abduction angle in their nondominant limb than their dominant limb (P = .01, ηp2 = 0.107) and the nondominant limb of athletes without a concussion history (P = .02, ηp2 = 0.083) during jump landing. They also had less trunk lateral bending during cutting compared with athletes without a concussion history (P = .005, ηp2 = 0.126).
CONCLUSIONS: Our results indicated landing biomechanics are different between female athletes with and those without a concussion history. This finding may be due to impairments in neuromuscular control postconcussion that may ultimately increase the risk of subsequent lower extremity injury, although further research is warranted given the cross-sectional nature of our study.

Elicitors, irrigation regimes and harvest times influence the content, yield and compound of the essential oil (EO) in Salvia officinalis (sage), through changes in biomass dynamics and biosynthetic pathways. A two-year field experiment was conducted to determine if foliar application of putrescine under optimum and deficit stress conditions would favorably affect EO yield, content and profile of sage harvested in spring and summer. The response of dry weight, EO yield and content, myrcene and borneol concentrations to irrigation regime and putrescine concentration can be expressed by a quadratic model. The maximum dry weight (182.63 g m-2) and EO yield (1.68 g m-2) were predicted under irrigation regimes of 9.06% and 27.75% available soil water depletion (ASWD), respectively. The highest EO content (1.05%) was predicted under 3.04 mM of putrescine. Based on results obtained from GC/MS analyses, 25 compounds (mostly monoterpenes) were identified in the EO of sage. Among EO compounds, α-thujone (54.08%), 1, 8-cineole (17.87%), pinocarvone (14.30%), β-thujone (7.97%) and camphor (8.76%) in turn were the most abundant. The concentration of myrcene was higher in spring than summer under the irrigation regimes of 60% and 80% ASWD. The myrcene concentration reached its maximum (4.53%) under the irrigation regime of 86.5% ASWD. The irrigation regimes of 48.03% and 45.6% ASWD caused the highest borneol concentrations of 1.47% and 1.41% by application of 1.5 mM and 2.25 mM putrescine, respectively. All treatments tested on sage, particularly harvest time, can play an important role in the improvement of EO quality and quantity. Averaged over both years, the irrigation regime of nearly 30% ASWD resulted in the highest EO yield harvested with greater quantity and better quality in summer. The EO content and quality changed slightly with the application of putrescine, without significant effect on yield.

Interest in causality is growing in sustainability science and it has been argued that a multiplicity of approaches is needed to account for the complexities of social-ecological dynamics. However, many of these approaches operate within perspectives that establish a separation between what has causal agency and all the rest, which is relegated to the role of background conditions. We argue that the distinction between causal elements and background conditions is by no means a necessary one, and that the causal agency of background conditions is worthy of investigation. We argue that such conditions correspond to what Karen Barad has called a \"cut\": a specific determination of the world (or part of it) respective to another part, for which it becomes intelligible. In this sense, most approaches to causality so far operate from \"within\" particular cuts. To illustrate this, we focus on the paradigmatic case of the Baltic cod collapse in the eighties. This case has been extensively studied, and overfishing has been identified as a key cause explaining the collapse. We dig deeper into the conditions which characterized fishing practices in the run-up to the collapse and uncover the separation between the social and the ecological that they enforce by encouraging policies to increase productivity under the rationale of national \"development\". We then re-examine the case from a process-relational perspective, rejecting the separation of nature from society. A process-relational perspective allows us to consider relations as constitutive of processes through which what exists becomes determinate. For this purpose we use the concepts of intra-action (co-constitution of processes) and of performativity (determination of language and matter within processes). We complete our conceptual framework by drawing inspiration from pragmatist philosophers and suggest that the concept of intuition can constitute an alternative to untangle causal dynamics and explain social-ecological phenomena beyond the cause/condition dichotomy. This article seeks to fulfil two objectives: firstly, to question the thick boundaries between conditions and causal elements that explain the processes in which social-ecological systems evolve; secondly, to provide a different approach to transforming a social-ecological system.

Basil is a plant known worldwide for its culinary and health attributes. It counts more than a hundred and fifty species and many more chemo-types due to its easy cross-breeds. Each species and each chemo-type have a typical aroma pattern and selecting the proper one is crucial for the food industry. Twelve basil varieties have been studied over three years (2018-2020), as have four different cuts. To characterize the aroma profile, nine typical basil flavour molecules have been selected using a gas chromatography-mass spectrometry coupled with an olfactometer (GC-MS/O). The concentrations of the nine selected molecules were measured by an ultra-fast CG e-nose and Principal Component Analysis (PCA) was applied to detect possible differences among the samples. The PCA results highlighted differences between harvesting years, mainly for 2018, whereas no observable clusters were found concerning varieties and cuts, probably due to the combined effects of the investigated factors. For this reason, the ANOVA Simultaneous Component Analysis (ASCA) methodology was applied on a balanced a posteriori designed dataset. All the considered factors and interactions were statistically significant (p < 0.05) in explaining differences between the basil aroma profiles, with more relevant effects of variety and year.

Carcass dissection is a more accurate method for determining the composition of a carcass; however, it is expensive and time-consuming. Techniques like VIA are of great interest once they are objective and able to determine carcass contents accurately. This study aims to evaluate the accuracy of a flexible VIA system to determine the weight and yield of the commercial value of carcass cuts of light lamb. Photos from 55 lamb carcasses are taken and a total of 21 VIA measurements are assessed. The half-carcasses are divided into six primal cuts, grouped according to their commercial value: high-value (HVC), medium-value (MVC), low-value (LVC) and all of the cuts (AllC). K-folds cross-validation stepwise regression analyses are used to estimate the weights of the cuts in the groups and their lean meat yields. The models used to estimate the weight of AllC, HVC, MVC and LVC show similar results and a k-fold coefficient of determination (k-fold-R2) of 0.99 is achieved for the HVC and AllC predictions. The precision of the weight and yield of the three prediction models varies from low to moderate, with k-fold-R2 results between 0.186 and 0.530, p < 0.001. The prediction models used to estimate the total lean meat weight are similar and low, with k-fold-R2 results between 0.080 and 0.461, p < 0.001. The results confirm the ability of the VIA system to estimate the weights of parts and their yields. However, more research is needed on estimating lean meat yield.

Sweet basil (Ocimum basilicum L.) is an economically important leafy vegetable especially in Mediterranean countries. In Italian gastronomy, the large elliptical leaves of the Genovese type are mostly used for the well-known pesto sauce, and almost all (>90%) professional production is for the food industry. The growing demand for fresh leaves with standardized technological and sensory characteristics has prompted basil producers to adopt advanced cultivation methods such as the floating raft system (FRS). The aim of this study was to evaluate the productive, qualitative, and physiological performance of three Genovese basil cultivars (\"Aroma 2,\" \"Eleonora,\" and \"Italiano Classico\") in two successive harvests and at two densities (159 and 317 plants m-2). Caffeic, chicoric, rosmarinic, and ferulic acid were determined through the high-performance liquid chromatography (HPLC) system, whereas the extraction and quantification of the volatile organic compounds (VOCs) were performed by solid-phase microextraction (SPME) and gas chromatography coupled to a mass spectrometer (GC/MS). \"Aroma 2\" showed the highest fresh yield and photosynthetic rate together with the lowest nitrate content. For all the tested cultivars, the higher density, while reducing the number of leaves per plant, resulted in higher fresh and dry production per unit area, without altering the aroma profile. Successive harvests resulted in a significant increase in both the yield (37.5%) and the total phenolic acids (75.1%) and favored Eucalyptol and 1-octen-3-ol accumulation (+25.9 and +15.1%, respectively). The here presented comprehensive and multifactorial assessment of the productive and qualitative response of basil provides evidence of the positive effects (from biomass to specialized metabolites) that can be obtained from the management of the pre-harvest factors in soilless cultivation. In addition, it also highlights the role and constraints of the genetic factor in the observed response. We also discuss the implications of our work considering the impact for the food processing industry. Future research may explore the phenolic acids accumulation as a possible fortification means to extend the pesto sauce shelf life, reducing the need of added antioxidants and thermal processing.

UNASSIGNED: Lucerne is a perennial legume forage, which can produce multiple cuts in 1 year. Microelements play fundamental roles in the function, maintenance and adaptation to the environment for lucerne growth. However, the role of the accumulation of copper (Cu), iron (Fe), manganese (Mn) and Zinc (Zn), which vary with lucerne ages or cuts, has not been previously determined. Therefore, a hypothesis on the Cu, Fe, Mn and Zn in lucerne varying with age and cut was tested.
UNASSIGNED: A total of 11, 8, 5, 4 and 1 year old lucerne (Medicago sativa Longdong) were selected as the material (until 2012 year), and samples were taken as three cuts at the cutting periods (early flowering stage) in 2012. Then, the contents and yields of Cu, Fe, Mn and Zn in lucerne were measured and calculated.
UNASSIGNED: The highest contents of Cu, Fe, Mn and Zn in lucerne were found in the 1 year old among the five ages, at the 3rd cut compared to the other two cuts, and in the leaf among the three organs. The highest yields of Cu, Fe, Mn and Zn were found in the older ages (11 and 8 years old), at the 3rd cut, and in the root among the three organs. The most positive correlations were found between contents, yields and biomass.
UNASSIGNED: The hypothesis was supported by the results. And the contents and yields of lucerne Cu, Fe, Mn and Zn were affected by the age, cut and organ. Furthermore, the yields of lucerne Cu, Fe, Mn and Zn were determined by their contents and lucerne biomass.