中东-亚洲小牛1(MEAM1)和地中海(MED)是甘薯粉虱中最具侵入性的两个成员,烟粉虱,隐蔽物种复合物,是斑马病毒的有效载体。烟粉虱MEAM1是美国东南部露地蔬菜作物中begomovirus的主要载体。然而,最近B.tabaciMED也在佛罗里达州和乔治亚州的温室外的景观中被发现。这项研究比较了美国东南部流行的一种旧世界(OW)和两种新世界(NW)双生病毒的传播效率,viz.,番茄黄化卷叶病毒(TYLCV),葫芦叶皱病毒(CuLCrV),和西达金花叶病毒(SiGMV)之间的B.tabaciMEAM1和B.tabaciMED。烟粉虱MEAM1有效传输TYLCV,CuLCrV,或者SiGMV,而B.tabaciMED仅传输TYLCV。在72小时的获取访问期后,烟粉虱MED的OWTYLCV的获取和保留百分比显着高于烟粉虱MEAM1。相比之下,B.tabaciMEAM1获得并保留了显着更多的NW双向双歧病毒,CuLCrV或SiGMV,比B.tabaciMED。粉虱内部组织中病毒DNA的定量分析(qPCR)显示,与烟粉虱MEAM1相比,烟粉虱MED中CuLCrV或SiGMV的积累减少。荧光原位杂交(FISH)显示CuLCrV或SiGMV在烟粉虱MED和烟粉虱MEAM1的中肠中的定位。然而,CuLCrV或SiGMV的定位仅在烟粉虱MEAM1的初级唾液腺中观察到,而不是烟粉虱MED。在烟粉虱MEAM1和烟粉虱MED的所有内部组织中均观察到TYLCV定位。总的来说,结果表明,烟粉虱MEAM1和烟粉虱MED都是OWTYLCV的有效载体。然而,对于NWBegomovirus,CuLCrV和SiGMV,B.tabaciMEAM1似乎是一个更好的载体。
Middle East-Asia Minor 1 (MEAM1) and Mediterranean (MED) are two of the most invasive members of the sweetpotato whitefly, Bemisia tabaci, cryptic species complexes and are efficient vectors of begomoviruses. Bemisia tabaci MEAM1 is the predominant vector of begomoviruses in open-field vegetable crops in the southeastern United States. However, recently B. tabaci MED also has been detected in the landscape outside of greenhouses in Florida and Georgia. This study compared the transmission efficiency of one Old-World (OW) and two New-World (NW) begomoviruses prevalent in the southeastern United States, viz., tomato yellow leaf curl virus (TYLCV), cucurbit leaf crumple virus (CuLCrV), and sida golden mosaic virus (SiGMV) between B. tabaci MEAM1 and B. tabaci MED. Bemisia tabaci MEAM1 efficiently transmitted TYLCV, CuLCrV, or SiGMV, whereas B. tabaci MED only transmitted TYLCV. Percent acquisition and retention of OW TYLCV following a 72 h acquisition access period was significantly higher for B. tabaci MED than B. tabaci MEAM1. In contrast, B. tabaci MEAM1 acquired and retained significantly more NW bipartite begomoviruses, CuLCrV or SiGMV, than B. tabaci MED. Quantitative analysis (qPCR) of virus DNA in whitefly internal tissues revealed reduced accumulation of CuLCrV or SiGMV in B. tabaci MED than in B. tabaci MEAM1. Fluorescent in situ hybridization (FISH) showed localization of CuLCrV or SiGMV in the midgut of B. tabaci MED and B. tabaci MEAM1. However, localization of CuLCrV or SiGMV was only observed in the primary salivary glands of B. tabaci MEAM1 and not B. tabaci MED. TYLCV localization was observed in all internal tissues of B. tabaci MEAM1 and B. tabaci MED. Overall, results demonstrate that both B. tabaci MEAM1 and B. tabaci MED are efficient vectors of OW TYLCV. However, for the NW begomoviruses, CuLCrV and SiGMV, B. tabaci MEAM1 seems to a better vector.