Leptomias群代表了青藏高原及其邻近地区最多样化的象鼻虫分类群之一。尽管隐藏的多样性的潜力,对该分类群的物种多样性进行的综合研究相对较少。在这项研究中,我们使用全面的DNA条形码数据集进行了Leptomias组物种的DNA条形码分析,该数据集包括代表54种形态物种的476条序列.在数据集中,我们的实验室贡献了474个序列,和390个序列新产生的这项研究。形态物种和属之间的平均木村2参数距离为0.76%和19.15%,分别。在94.4%的物种中,最小种间距离超过最大种内距离,表明在大多数Leptomias组物种中存在条形码缺口。条形码间隙自动发现的应用,通过自动分区组装物种,条形码索引号,贝叶斯泊松树过程,jMOTU,邻居连接树方法揭示了代表单个物种的45、45、63、54和55个不同的簇,分别。此外,总共有四个形态物种,Leptomiaskangmorensis,L.midlineatus,L.siahus,和L.sp.9RL,被发现被分配给多个子分支,表明地理差异和神秘多样性的存在。我们的研究结果表明,青藏高原具有较高的Leptomias群物种多样性,在未来的研究中,必须使用综合分类学方法研究某些形态物种中的隐秘物种。此外,本文提出的DNA条形码参考文库的构建建立了一个强大的基础数据集,以支持即将进行的象鼻虫分类学研究,系统发育学,生态学,和进化。
The Leptomias group represents one of the most diverse taxonomic group of weevils in the Qinghai-Tibet Plateau and its adjacent areas. Despite the potential of hidden diversity, relatively few comprehensive studies have been conducted on species diversity in this taxonomic group. In this study, we performed DNA barcoding analysis for species of the Leptomias group using a comprehensive DNA barcode dataset that included 476 sequences representing 54 morphospecies. Within the dataset, our laboratory contributed 474 sequences, and 390 sequences were newly generated for this study. The average Kimura 2-parameter distances among morphospecies and genera were 0.76% and 19.15%, respectively. In 94.4% of the species, the minimum interspecific distances exceeded the maximum intraspecific distances, indicating the presence of barcode gaps in most species of Leptomias group. The application of Automatic Barcode Gap Discovery, Assemble Species by Automatic Partitioning, Barcode Index Number, Bayesian Poisson tree processes, jMOTU, and Neighbor-joining tree methods revealed 45, 45, 63, 54, and 55 distinct clusters representing single species, respectively. Additionally, a total of four morphospecies, Leptomias kangmarensis, L. midlineatus, L. siahus, and L. sp.9RL, were found to be assigned to multiple subclade each, indicating the geographical divergences and the presence of cryptic diversity. Our findings of this study demonstrate that Qinghai-Tibet Plateau exhibits a higher species diversity of the Leptomias group, and it is imperative to investigate cryptic species within certain morphospecies using integrative taxonomic approaches in future studies. Moreover, the construction of a DNA barcode reference library presented herein establishes a robust foundational dataset to support forthcoming research on weevil taxonomy, phylogenetics, ecology, and evolution.