一系列结构分析,也就是说,在电子显微镜水平上对整个细胞进行定量和三维结构分析,已经在Exophialadermatitidis中单独实现,酿酒酵母,结核分枝杆菌,Myojin螺旋细菌,和大肠杆菌。在这些分析中,通过冷冻固定和快速冷冻替代处理样品细胞,导致通过透射电子显微镜检查的连续超薄切片上的超微结构的精美保存。在本文中,非致病性耻垢分枝杆菌的结构分析,基本名称耻垢分枝杆菌,已执行。作为高致病性结核分枝杆菌的替代品,耻垢分枝杆菌经常被用于分子生物学实验和实验性结核病。比较同一属的两个物种一直是一项任务,分枝杆菌,通过结构分析。七个耻垢分枝杆菌细胞切成连续的超薄切片,and,完全,通过透射电子显微镜检查220个连续超薄切片。测量细胞分布,包括细胞长度,细胞和细胞质的直径,外膜和质膜的表面积,整个细胞的体积,周质,和细胞质,每0.1fl细胞质的核糖体总数和密度。这些数据是基于透射电子显微镜图像中精细保存的单细胞结构的直接测量和枚举,并且不是基于生化或分子生物学间接数据的计算或假设。所有的测量结果都是耻垢分枝杆菌,除了单元格长度,明显高于结核分枝杆菌。此外,这些数据可能解释了耻垢分枝杆菌比结核分枝杆菌生长更快,并有助于理解其结构特性,与结核分枝杆菌有很大不同,关于抗原性的表达,耐酸牢度,以及耐药机制与靶标与相应药物的比例有关。此外,从低温透射电子显微镜检查获得的数据用于支持结构体分析的有效性。最后,我们的数据有力地支持了新的分枝杆菌属的最新建立,将耻垢分枝杆菌分类为基本名称。
A series of structome analyses, that is, quantitative and three-dimensional structural analysis of a whole cell at the electron microscopic level, have already been achieved individually in Exophiala dermatitidis, Saccharomyces cerevisiae, Mycobacterium tuberculosis, Myojin spiral bacteria, and Escherichia coli. In these analyses, sample cells were processed through cryo-fixation and rapid freeze-substitution, resulting in the exquisite preservation of ultrastructures on the serial ultrathin sections examined by transmission electron microscopy. In this paper, structome analysis of non pathogenic Mycolicibacterium smegmatis, basonym Mycobacterium smegmatis, was performed. As M. smegmatis has often been used in molecular biological experiments and experimental tuberculosis as a substitute of highly pathogenic M. tuberculosis, it has been a task to compare two species in the same genus, Mycobacterium, by structome analysis. Seven M. smegmatis cells cut into serial ultrathin sections, and, totally, 220 serial ultrathin sections were examined by transmission electron microscopy. Cell profiles were measured, including cell length, diameter of cell and cytoplasm, surface area of outer membrane and plasma membrane, volume of whole cell, periplasm, and cytoplasm, and total ribosome number and density per 0.1 fl cytoplasm. These data are based on direct measurement and enumeration of exquisitely preserved single cell structures in the transmission electron microscopy images, and are not based on the calculation or assumptions from biochemical or molecular biological indirect data. All measurements in M. smegmatis, except cell length, are significantly higher than those of M. tuberculosis. In addition, these data may explain the more rapid growth of M. smegmatis than M. tuberculosis and contribute to the understanding of their structural properties, which are substantially different from M. tuberculosis, relating to the expression of antigenicity, acid-fastness, and the mechanism of drug resistance in relation to the ratio of the targets to the corresponding drugs. In addition, data obtained from cryo-transmission electron microscopy examination were used to support the validity of structome analysis. Finally, our data strongly support the most recent establishment of the novel genus Mycolicibacterium, into which basonym Mycobacterium smegmatis has been classified.