壳聚糖(CS),一种生物聚合物,由于其生物相容性和可生物降解性,在骨再生中具有重要的潜力。虽然甲壳类动物衍生的CS通常用于研究,真菌衍生的CS因其在骨再生应用中同样有效的特性而受到越来越多的关注。这里,我们研究了真菌(MDC)和甲壳动物(ADC)衍生的CS支架的物理化学和生物学特性,其中嵌入了不同浓度的磷酸三钙矿物质(TCP),即,0(wt)%:ADC/MDC-1,10(wt)%:ADC/MDC-2,20(wt)%:ADC/MDC-3和30(wt)%:ADC/MDC-4。缺乏TCP矿物质的ADC-1和MDC-1冻干支架呈现47.3±1.2mV和55.1±1.6mV的最高zeta电位,分别。扫描电子显微镜显示出明显的区别,与ADC支架类型所表现出的多孔形态相比,MDC支架表现出条纹状结构微结构。关于4周的脚手架质量减少,MDC-1、MDC-2、MDC-3和MDC-4显示下降55.98±4.2%,40.16±3.6%,27.05±4.7%,和19.16±5.3%,分别。相反,ADC-1、ADC-2、ADC-3和ADC-4的质量减少35.78±5.1%,25.19±4.2%,20.23±6.3%,13.68±5.4%,分别。通过间接和直接的细胞毒性研究,通过体外骨髓间充质基质细胞(BMMSCs)附着评估支架的生物学性能,所有支架类型均无细胞毒性行为。MDC支架显示结果与ADC相当,其中两种CS类型表现出相似的理化性质。我们的数据表明,MDC支架可能是用于骨再生应用的ADC衍生支架的有效替代品。特别是对于10(wt)%的TCP浓度。
Chitosan (CS), a biopolymer, holds significant potential in bone regeneration due to its biocompatibility and biodegradability attributes. While
crustacean-derived CS is conventionally used in research, there is growing interest in fungal-derived CS for its equally potent properties in bone regenerative applications. Here, we investigated the physicochemical and biological characteristics of fungal (MDC) and
crustacean (ADC)-derived CS scaffolds embedded with different concentrations of tricalcium phosphate minerals (TCP), i.e., 0(wt)%: ADC/MDC-1, 10(wt)%: ADC/MDC-2, 20(wt)%: ADC/MDC-3 and 30(wt)%: ADC/MDC-4. ADC-1 and MDC-1 lyophilised scaffolds lacking TCP minerals presented the highest zeta potentials of 47.3 ± 1.2 mV and 55.1 ± 1.6 mV, respectively. Scanning electron microscopy revealed prominent distinctions whereby MDC scaffolds exhibited striation-like structural microarchitecture in contrast to the porous morphology exhibited by ADC scaffold types. With regard to the 4-week scaffold mass reductions, MDC-1, MDC-2, MDC-3, and MDC-4 indicated declines of 55.98 ± 4.2%, 40.16 ± 3.6%, 27.05 ± 4.7%, and 19.16 ± 5.3%, respectively. Conversely, ADC-1, ADC-2, ADC-3, and ADC-4 presented mass reductions of 35.78 ± 5.1%, 25.19 ± 4.2%, 20.23 ± 6.3%, and 13.68 ± 5.4%, respectively. The biological performance of the scaffolds was assessed through in vitro bone marrow mesenchymal stromal cell (BMMSCs) attachment via indirect and direct cytotoxicity studies, where all scaffold types presented no cytotoxic behaviours. MDC scaffolds indicated results comparable to ADC, where both CS types exhibited similar physiochemical properties. Our data suggest that MDC scaffolds could be a potent alternative to ADC-derived scaffolds for bone regeneration applications, particularly for 10(wt)% TCP concentrations.