背景:除草剂百草枯(PQ)具有剧毒,能够诱导严重的肺部炎症和氧化应激,导致肺纤维化和呼吸衰竭。先前的研究已经证明了与番红花相关的一系列药理作用。L(Cs)通过其抗炎,抗氧化和免疫调节特性。药理学研究支持在传统医学中广泛使用Cs治疗咳嗽和哮喘等呼吸系统疾病。研究目的:本研究旨在研究Cs提取物和吡格列酮(Pio)对肺部炎症的预防性影响。氧化应激,病理改变,与地塞米松(Dexa)相比,大鼠吸入PQ诱导的气管反应性。
方法:对照组(Ctrl)大鼠给予生理盐水气雾剂,其余6组每隔一天服用8次PQ气雾剂。在暴露于PQ期间,六个PQ暴露组每天都用其中之一进行治疗;单独的盐水,低剂量Cs,高剂量Cs,皮奥独自一人,Pio联合低剂量Cs,或者16天的德克萨。
结果:在PQ组中,超氧化物歧化酶(SOD)的水平,过氧化氢酶(CAT),支气管肺泡灌洗液(BALF)中的硫醇减少,MDA的水平,总WBC和微分WBC,肺组织肿瘤坏死因子(TNF-α)和白细胞介素10(IL-10)水平,气管对乙酰甲胆碱的反应性(TR)和肺部病理变化增强。测量的变量在所有治疗组中显示出显着的改善,除了Cs(L)中的几个变量。组合的Cs(L)+Pio显示出比单独的Cs(L)和Pio更高的效果。对于所有比较,p值<0.05至<0.001。
结论:结果显示Cs的预防效果与Dexa相当,Cs和Pio的潜在累加预防能力表明PPARγ受体的参与与Cs诱导的作用有关。
BACKGROUND: The herbicide paraquat (PQ) is highly toxic, capable of inducing severe lung inflammation and oxidative stress, resulting in lung fibrosis and respiratory failure. Previous research has demonstrated a range of pharmacological effects associated with Crocus sativus. L (Cs) through its anti-inflammatory, antioxidant and immunomodulatory properties. Pharmacological studies support the widespread use of Cs in traditional medicine to treat respiratory disorders such as coughs and asthma.
OBJECTIVE: This study aimed to investigate the preventive impact of Cs extract and pioglitazone (Pio) on lung inflammation, oxidative stress, pathological alterations, and tracheal reactivity induced by inhaled PQ in rats as compared to dexamethasone (Dexa).
METHODS: The control (Ctrl) group of rats was administered with saline aerosol, while the remaining six groups received PQ aerosol eight times every other day. The six PQ exposure groups were treated daily during the exposure period to PQ with either; saline alone, low dose Cs, High dose Cs, Pio alone, Pio combined with low dose Cs, or Dexa of 16 days.
RESULTS: In the PQ group, the levels of superoxide dismutase (SOD), catalase (CAT), and thiol in the bronchoalveolar lavage fluid (BALF) were declined whereas, the levels of MDA, total and differential WBC, and lung tissue levels of tumor necrosis factor (TNF-α) and Interleukin 10 (IL-10), tracheal responsiveness (TR) to methacholine and lung pathological changes were enhanced. The measured variables showed significant improvement in all treated groups, except for a few variables in Cs (L). The combined Cs (L) + Pio showed higher effects than Cs (L) and Pio alone. For all comparisons, p values were <0.05 to <0.001.
CONCLUSIONS: The results showed preventive effect of Cs comparable to that of Dexa and the potential additive preventive capabilities of the Cs and Pio indicate that the involvement of the PPARγ receptor is implicated in the effects induced by Cs.