虽然棒状杆菌属。可以定期与奶牛的亚临床和临床乳腺炎病例相关,关于他们在奶牛场的水库的知识很少。因此,样本在10次访问时收集,间隔14天,从床上用品(n=50),饮酒低谷(n=20),不同的步行区域(n=60),牛刷子(n=8),捕蝇(n=4),一个奶牛养殖场的牧场通道(n=9)以及挤奶衬里(n=80)和挤奶手套(n=20)。此外,在每次访问中收集来自所有泌乳奶牛(约200头)的四分之一的前乳样品。所有样品均接受微生物学检查,并使用MALDI-TOFMS鉴定培养的分离株。大多数棒状杆菌属。从牛奶中培养出来的食物也与住房环境和挤奶相关的生态位(C.淀粉样,C.confusum,C.车站,C.variabile,C.干燥症)或仅来自挤奶相关的壁龛(C.frankenforstense,C.pilosum,C.suicordis)。C.牛不是从任何环境生态位种植的,同时是牛奶样品中的优势物种。这项研究表明,许多棒状杆菌属。牛奶中存在的样品也可以从奶牛环境中分离出来。对于牛,与乳房内感染最相关的棒状杆菌物种,这表明环境水库没有什么相关性。
Although Corynebacterium spp. can be regularly associated with subclinical and clinical mastitis cases in dairy cows, knowledge on their reservoirs in dairy farms is sparse. Therefore, samples were collected at 10 visits with 14 day intervals from bedding material (n = 50), drinking troughs (n = 20), different walking areas (n = 60), cow brushes (n = 8), fly traps (n = 4), the passage to pasture (n = 9) as well as milking liners (n = 80) and milker gloves (n = 20) in one dairy cow farm. Additionally, quarter foremilk samples from all lactating cows (approximately 200) were collected at each visit. All samples underwent microbiological examination and cultured isolates were identified using MALDI-TOF MS. Most Corynebacterium spp. that were cultivated from milk were also isolated from the housing environment and milking-related niches (C. amycolatum, C. confusum, C. stationis, C. variabile, C. xerosis) or from milking-related niches only (C. frankenforstense, C. pilosum, C. suicordis). C. bovis was not cultivated from any environmental niche, while being the dominant species in milk samples. This study demonstrates that many Corynebacterium spp. present in milk samples can also be isolated from the cows’ environment. For C. bovis, the most relevant Corynebacterium species with regard to intramammary infections, it indicates that environmental reservoirs are of little relevance.