鸡被认为是研究多不饱和脂肪酸(PUFA)合成的理想模型物种,因为它具有适当的脂肪酸比例和丰富的PUFA含量,适合人类消费。然而,调节家禽PUFA合成的分子机制尚不清楚.这里,我们通过进行双荧光素酶报告基因分析,系统地探讨了鸡PUFA合成相关基因家族的转录调控活性。我们确定了鸡PUFA合成相关基因家族成员的核心启动子区域,包括ELOVL1,ELOVL2,ELOVL3,ELOVL4,ELOVL5,ELOVL6,ELOVL7,FADS1,FADS2,FADS6,SCD,SCD5。此外,这些基因的上游调控区中不同截短片段的相对荧光值的变化表明调控区的存在。此外,我们预测了与多个基因的核心启动子区域结合的转录因子,包括Sp1,NF-1,C/EBPalpha,等。这些发现为调节家禽PUFA合成的分子机制提供了基础,并为未来禽肉品质的潜在改善提供了新的科学见解。
Chicken is considered an ideal model species to study the synthesis of polyunsaturated fatty acids (PUFAs) due to its appropriate proportions of fatty acids and abundant content of PUFAs, suitable for human consumption. However, the molecular mechanisms regulating poultry PUFA synthesis remain unclear. Here, we systematically explored the transcriptional regulation activity of the gene family related to PUFA synthesis in chicken by carrying out the Dual-Luciferase Reporter Assay. We identified the core promoter regions of members of the chicken PUFA synthesis-related gene family, including ELOVL1, ELOVL2, ELOVL3, ELOVL4, ELOVL5, ELOVL6, ELOVL7, FADS1, FADS2, FADS6, SCD, and SCD5. Additionally, changes in relative fluorescence values of different truncated segments in the upstream regulatory region of these genes indicate the existence of regulatory regions. Furthermore, we predicted the transcription factors that bind to the identified core promoter regions of multiple genes, including Sp1, NF-1, C/EBPalpha, etc. These findings provide a basis for the molecular mechanisms regulating poultry PUFA synthesis and offer new scientific insight into the potential improvement of poultry meat quality in the future.