■挫伤型创伤性脑损伤(TBI)是全球范围内运动障碍和死亡的主要原因。虽然TBI后的有害后果受性别和肠道菌群失调的影响,TBI后共生肠道微生物群的性别特异性重要性不足。在这项研究中,我们以性别特异性方式研究了控制性皮质撞击(CCI)损伤对小鼠肠道微生物群特征的影响.
■我们使用抗生素治疗耗尽了雄性和雌性C57BL/6小鼠的肠道菌群。此后,雄性小鼠被雌性小鼠的肠道微生物群定植,反之亦然,采用粪便微生物移植(FMT)方法。使用立体定位撞击器(ImpactOne™)进行CCI手术。对于16SrRNA基因扩增子研究,在CCI(dpi)后3天收集小鼠的粪便丸剂。
■CCI操作的雄性和雌性小鼠在Akkermansia属中表现出明显的改变,Alistipes,拟杆菌,梭菌属,乳酸菌,普雷沃氏菌,和Ruminococus.在物种层面,在雌性小鼠中观察到较少的瑞士乳杆菌和仓鼠乳杆菌,暗示性别特异性细菌疗法在CCI诱导的神经功能缺损中的重要性。从雌性供体小鼠到雄性小鼠的FMT表现出Alistipes属的增加,乳酸菌,和Ruminococcus以及酸化拟杆菌和gnavusRuminococus的物种。来自雄性供体的雌性FMT受体小鼠在乳杆菌属和瑞士乳杆菌属中表现出热潮,仓鼠乳杆菌,和Prevotellacopri.这些结果表明,CCI后神经系统并发症可能受到雄性和雌性小鼠肠道微生物群差异的影响。
UNASSIGNED: Contusion type of traumatic brain injury (TBI) is a major cause of locomotor disability and mortality worldwide. While post-TBI deleterious consequences are influenced by gender and gut dysbiosis, the sex-specific importance of commensal gut microbiota is underexplored after TBI. In this study, we investigated the impact of controlled cortical impact (CCI) injury on gut microbiota signature in a sex-specific manner in mice.
UNASSIGNED: We depleted the gut microflora of male and female C57BL/6 mice using antibiotic treatment. Thereafter, male mice were colonized by the gut microbiota of female mice and vice versa, employing the fecal microbiota transplantation (FMT) method. CCI surgery was executed using a stereotaxic impactor (Impact One™). For the 16S rRNA gene amplicon study, fecal boli of mice were collected at 3 days post-CCI (dpi).
UNASSIGNED: CCI-operated male and female mice exhibited a significant alteration in the genera of Akkermansia, Alistipes, Bacteroides, Clostridium, Lactobacillus, Prevotella, and Ruminococcus. At the species level, less abundance of Lactobacillus helveticus and Lactobacillus hamsteri was observed in female mice, implicating the importance of sex-specific bacteriotherapy in CCI-induced neurological deficits. FMT from female donor mice to male mice displayed an increase in genera of Alistipes, Lactobacillus, and Ruminococcus and species of Bacteroides acidifaciens and Ruminococcus gnavus. Female FMT-recipient mice from male donors showed an upsurge in the genus Lactobacillus and species of Lactobacillus helveticus, Lactobacillus hamsteri, and Prevotella copri. These results suggest that the post-CCI neurological complications may be influenced by the differential gut microbiota perturbation in male and female mice.