外科污染,创伤性,和具有微生物的慢性伤口对成功的伤口愈合提出了挑战。在目前的体外研究中,一种合成电纺纤维基质(SEFM),用于治疗慢性,外科,和创伤性伤口接受USP(美国药典)<51>抗微生物效力测试以确定其针对通常在不愈合伤口中发现的各种微生物的体外效力。SEFM在薄片(s-SEFM)和微粉化形式(m-SEFM)中针对大肠杆菌进行了测试,铜绿假单胞菌,金黄色葡萄球菌,巴西曲霉,白色念珠菌,变形杆菌,和粪肠球菌.在第7、14和28天按照USP<51>标准进行测试。s-SEFM和m-SEFM均满足所有微生物的USP<51>接受标准。s-SEFM获得的结果表明,对大肠杆菌的减少>1-log10,金黄色葡萄球菌,铜绿假单胞菌,P.奇迹,E.粪便,在第7天和白色念珠菌;>3-log10减少,在第14天和第28天未检测到这些微生物,并且在第7、14和28天对巴西念珠菌的初始接种量没有增加。对于m-SEFM获得的结果证明>3-log10减少,在第7天没有可检测的微生物。在该研究中观察到的结果表明,根据USP<51>测试,SEFM在体外有效抑制细菌和真菌生长和定植。
Contamination of surgical, traumatic, and chronic wounds with microorganisms presents a challenge to successful wound healing. In the present in vitro study, a synthetic electrospun fiber matrix (SEFM) cleared for use in the management of chronic, surgical, and traumatic wounds underwent USP (United States Pharmacopeia) <51> Antimicrobial Effectiveness Testing to determine its in vitro effectiveness against various microorganisms commonly found in non-healing wounds. The SEFM was tested in both sheet (s-SEFM) and micronized form (m-SEFM) against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Aspergillus brasiliensis, Candida albicans, Proteus mirabilis, and Enterococcus faecalis. Testing was performed per the USP <51> standard on days 7, 14, and 28. Both the s-SEFM and m-SEFM met the USP <51> acceptance criteria for all microorganisms. The results obtained for s-SEFM demonstrated >1-log10 reduction against E. coli, S. aureus, P. aeruginosa, P. mirabilis, E. faecalis, and C. albicans at day 7; >3-log10 reduction with no detection of these microbes at days 14 and 28, and no increase from initial inoculum at days 7, 14, and 28 against A. brasiliensis. The results obtained for m-SEFM demonstrated >3-log10 reduction with no detectable microorganisms at day 7. The results observed in this study indicate that the SEFM is effective in vitro at inhibiting bacterial and fungal growth and colonization per USP <51> testing.