目的:大多数牙髓疾病是细菌介导的牙髓污染引起的炎症或坏死过程。尽管在获得针对持续性感染的更有效的抗菌策略方面正在取得重大进展,大多数研究缺乏具有代表性的模型来测试其抗菌作用,其结果无法及时转化为临床实践.因此,这项研究旨在完善结合人类牙齿的离体牙髓生物膜模型,计算机引导设计和3D打印,以获得更具可重复性和可预测性的模型。
方法:使用三种不同的方法切割单齿:手持(HCC),机械精度(MPC)和计算机辅助引导切割(CGC)。然后,块被重新组装。在尺寸公差方面评估了不同的模型制剂,表面分析,液体密封性和粪肠球菌生物膜发展21天,通过代谢测定和共聚焦显微镜进行了研究。然后,使用不同的商业消毒方法验证了所提出的模型。
结果:与HHC和MPC相比,CGC表现出明显更低的偏差和表面无缺陷,导致优越的液体密封性。同样,在所有条件下都观察到具有高代谢活性和活力的成熟生物膜,CGC显示出最低的变异。关于模型验证,所有抗菌治疗都导致标准2D模型中细菌的完全根除,而商业治疗在拟议的离体模型中表现出不同水平的疗效,从适度降低代谢活性到完全消除生物膜。
结论:新的指导方法代表了一种更可靠的,标准化,和可重复的模型,用于评估牙髓消毒治疗。
结论:在抗菌治疗发展过程中,具有挑战性的3D模型使用牙齿基质来测试抗菌治疗新的引导方法代表了一种更可靠的,标准化,和可重复的模型,用于评估牙髓消毒治疗。
OBJECTIVE: Most endodontic diseases are bacterium-mediated inflammatory or necrotic process induced by contaminated dental pulp. Although great advances are being performed to obtain more efficient antibacterial strategies for persistent infections, most studies lack of representative models to test their antibacterial effects and their outcomes cannot be promptly translated to clinical practice. Therefore, this study aimed to refine an ex vivo endodontic biofilm model combining human tooth, computer guided design and 3D printing to obtain a more reproducible and predictable model.
METHODS: Monoradicular teeth were cut using three different methods: hand-held (HCC), mechanical precision (MPC) and computer aid guided cutting (CGC). Then, blocks were reassembled. The different model preparations were assessed in terms of dimensional tolerance, surface analysis, liquid tightness and Enterococcus faecalis biofilm development for 21 days, which was studied by metabolic assays and confocal microscopy. Then, the proposed model was validated using different commercial disinfecting treatments.
RESULTS: CGC exhibited significantly lower deviation and surface without defects compared to HHC and MPC, leading to superior liquid tightness. Similarly, mature biofilms with high metabolic activity and vitality were observed in all conditions, CGC showing the lowest variation. Regarding the model validation, all antibacterial treatments resulted in the complete eradication of bacteria in the standard 2D model, whereas commercial treatments exhibited varying levels of efficacy in the proposed ex vivo model, from moderately reduction of metabolic activity to complete elimination of biofilm.
CONCLUSIONS: The novel guided approach represents a more reliable, standardized, and reproducible model for the evaluation of endodontic disinfecting therapies.
CONCLUSIONS: During antibacterial treatment development, challenging 3D models using teeth substrates to test antibacterial treatments novel guided approach represents a more reliable, standardized, and reproducible model for the evaluation of endodontic disinfecting therapies.