阿兰巴斯病(A.Alphylla),是一种番石榴科的物种,在我国西北地区广泛分布,具有较高的药理价值和生态功能。然而,生长特征知之甚少,生物农药的发展阻碍了其产业发展。这里,我们探索了A.allypla的再生能力。为此,在3月底发芽之前,对多年生树枝的二级分支进行了不同长度的修剪。四种处理方法分别是不割草(M0)和割草1/3、2/3和多年生分支的二级分支的整个长度(分别为M1-M3)。接下来,为了评估割草后的补偿性生长,每30天记录新的同化枝相关性状,并记录最终生物量。修剪过的植物比未修剪过的植物显示出更大的同化枝生长速率。此外,随着割草程度的增加,同化枝条的生长速率和最终生物量呈下降趋势,响应M1的生长速率和最终生物量最大。为了评估割草后补偿性生长的机制,对M0和M1处理进行了动态(修剪后0,1,5和8天)植物激素靶向代谢组学和转录组学的组合.总的来说,共检测到26种植物激素代谢产物,6与对照相比,割草后显着增加:吲哚-3-乙酰基-L-缬氨酸甲酯,吲哚-3-羧酸,吲哚-3-甲醛,赤霉素A24、赤霉素A4和顺式(+)-12-氧代-植物二烯酸。此外,在修剪的植物和对照之间检测到2,402个差异表达的基因。通过基于割草后表达趋势的聚类分析和每个聚类的基因本体分析相结合,鉴定出18个与生长素代谢相关的基因,其中6个与生长素合成显著相关。我们的发现表明,适当的割草可以促进A.aphylla的生长,受生长素代谢途径调节,并为A.aphylla的工业价值开发奠定了基础。
Anabasis aphylla (A. aphylla), a species of the Amaranthaceae family, is widely distributed in northwestern China and has high pharmacological value and ecological functions. However, the growth characteristics are poorly understood, impeding its industrial development for biopesticide development. Here, we explored the regenerative capacity of A. aphylla. To this end, different lengths of the secondary branches of perennial branches were mowed at the end of March before sprouting. The four treatments were no mowing (M0) and mowing 1/3, 2/3, and the entire length of the secondary branches of perennial branches (M1-M3, respectively). Next, to evaluate the compensatory growth after mowing, new assimilate branches\' related traits were recorded every 30 days, and the final biomass was recorded. The mowed plants showed a greater growth rate of assimilation branches than un-mowed plants. Additionally, with the increasing mowing degree, the growth rate and the final biomass of assimilation branches showed a decreasing trend, with the greatest growth rate and final biomass in response to M1. To evaluate the mechanism of the compensatory growth after mowing, a combination of dynamic (0, 1, 5, and 8 days after mowing) plant hormone-targeted metabolomics and transcriptomics was performed for the M0 and M1 treatment. Overall, 26 plant hormone metabolites were detected, 6 of which significantly increased after mowing compared with control: Indole-3-acetyl-L-valine methyl ester, Indole-3-carboxylic acid, Indole-3-carboxaldehyde, Gibberellin A24, Gibberellin A4, and cis (+)-12-oxo-phytodienoic acid. Additionally, 2,402 differentially expressed genes were detected between the mowed plants and controls. By combining clustering analysis based on expression trends after mowing and gene ontology analysis of each cluster, 18 genes related to auxin metabolism were identified, 6 of which were significantly related to auxin synthesis. Our findings suggest that appropriate mowing can promote A. aphylla growth, regulated by the auxin metabolic pathway, and lays the foundation for the development of the industrial value of A. aphylla.