While multiple studies have focused on the motivations surrounding antibiotic prescription among veterinarians, little is known about companion animal owners\' knowledge, attitude and perception (KAP) regarding the topic. A nationwide survey directed toward Portuguese dog and cat owners was conducted online and at veterinary practices to characterize their KAP regarding antibiotics. After database curation, a total of 423 valid submissions were considered. Although 97.9% of respondents stated that they knew what an antibiotic was, 23.5% and 19.2% answered that they were used to treat viral and fungal infections, respectively. Antimicrobial effectiveness was favored over cost when 87.7% of owners agreed they would prefer to spend more money to identify the appropriate antibiotic. Around 87% of respondents recognized antibiotic resistance as a significant health problem and 74.6% strongly agreed/somewhat agreed that antibiotic use in pets may contribute to resistance development. However, only 25.3% recognized that this could promote resistance dissemination, showing little awareness of the interconnection between human and animal health. Moreover, 55.6% of respondents were neutral when asked whether antibiotics used in veterinary medicine were also important for humans. These findings suggest that communication between veterinarians and pet owners can still be improved to further clarify the impact that antibiotic use has in pets from a One-Health perspective, also enabling antimicrobial stewardship interventions.

Although many companion animal (or \"pet\") owners report that their relationships with their pets are important, we know little about how animal ownership duration and animal life stage are related to relationship quality. In a sample of 1303 dog and cat owners, the present research explored the associations between relationship duration, pet life stage (puppy/kitten, young adult, mature adult, and senior), and four markers of relationship quality: pet-related self-expansion, perceived pet responsiveness, perceived pet insensitivity, and human-animal bond. We found that relationship duration was negatively and linearly associated with self-expansion for both dog and cat owners. Results comparing relationship quality markers across pet life stages revealed that for both dog and cat owners, self-expansion was higher for owners of younger animals compared to owners of older animals. There were no significant effects for perceived pet responsiveness, perceived pet insensitivity, or human-animal bond based on relationship duration or animal life stage. These findings indicate that the duration of the relationship with one\'s pet and animal life stage have implications for how much people feel a pet helps them grow, whereas other markers of human-pet relationship quality likely vary based on other experiences.

The interplay of regulatory T cells (Tregs) within the tumour microenvironment presents a significant challenge in anticancer immunotherapy. This study investigates the potential of Treg blockade to enhance the efficiency of effector T cells. Two distinct treatment cocktails were examined: 3p-hpRNA (5\' triphosphate hairpin RNA) combined with unmethylated CpG oligonucleotide (CpG); CpG in combination with OX40 receptor-specific monoclonal antibody (anti-OX40). Treatment efficacy was assessed using a murine model of kidney adenocarcinoma.Renca cells (renal cortical cells with adenocarcinoma) were subcutaneously engrafted in 30 BALB/c mice, then animals were allocated into three treatment groups: Group 1: CpG+anti-OX40, Group 2: CpG+3p-hpRNA, Group 3: untreated control. Treatment efficacy was evaluated based on tumour growth, the occurrence of metastases and overall survival.On day 28 post-implantation, experiments had to be terminated due to tumour progression. Although comparisons of survival times and primary tumour sizes thus became inconsequential, histological examinations provided valuable insights. We observed distinct variations in primary tumour characteristics among the different groups: Groups 1 and 2 displayed demarcations, while Group 3 exhibited diffuse tumours with necrosis. Lung metastases were evident in 70% of untreated mice, whereas none were observed in either of the treated groups.Our findings instil confidence in the potential efficacy of the treatments, thereby laying a solid foundation for future investigations.

Companion animals in veterinary medicine develop multiple naturally occurring diseases analogous to human conditions. We previously reported a comprehensive review on the feasibility, safety, and biologic activity of using novel stem cell therapies to treat a variety of inflammatory conditions in dogs and cats (2008-2015) [1]. The purpose of this review is to provide an updated summary of current studies in companion animal disease models that have evaluated stem cell therapeutics that are relevant to human disease. Here we have reviewed the literature from 2015 to 2023 for publications on stem cell therapies that have been evaluated in companion animals, including dogs, cats, and horses. The review excluded case reports or studies performed in experimentally induced models of disease, studies involving cancer, or studies in purpose-bred laboratory species such as rodents. We identified 45 manuscripts meeting these criteria, an increase from 19 that were described in the previous review [1]. The majority of studies were performed in dogs (n=28), with additional studies in horses (n=9) and cats (n=8). Disease models included those related to musculoskeletal disease (osteoarthritis, tendon/ligament injury), neurologic disease (canine cognitive dysfunction, intervertebral disc disease, spinal cord injury) gingival/dental disease (gingivostomatitis), dermatologic disease (atopic dermatitis), chronic multi-drug resistant infections, ophthalmic disease (keratoconjunctivitis sicca, eosinophilic keratitis, immune mediated keratitis), cardiopulmonary disease (asthma, degenerative valve disease, dilated cardiomyopathy), gastrointestinal disease (inflammatory bowel disease, chronic enteropathy) and renal disease (chronic kidney disease). The majority of studies reported beneficial responses to stem cell treatment, with the exception of those related to more chronic processes such as spinal cord injury and chronic kidney disease. However, it should also be noted that 22 studies were open-label, baseline-controlled trials and only 12 studies were randomized and controlled, making overall study interpretation difficult. As noted in the previous review, improved regulatory oversight and consistency in manufacturing of stem cell therapies is needed. Enhanced understanding of the temporal course of disease processes using advanced -omics approaches may further inform mechanisms of action and help define appropriate timing of interventions. Future directions of stem cell-based therapies could include use of stem-cell derived extracellular vesicles, or cell conditioning approaches to direct cells to specific pathways that are tailored to individual disease processes and stages of illness.

Both n-6 and n-3 fatty acids (FA) have numerous significant physiological roles for mammals. The interplay between these families of FA is of interest in companion animal nutrition due to the influence of the n-6:n-3 FA ratio on the modulation of the inflammatory response in disease management and treatment. As both human and animal diets have shifted to greater consumption of vegetable oils rich in n-6 FA, the supplementation of n-3 FA to canine, feline, and equine diets has been advocated for. Although fish oils are commonly added to supply the long-chain n-3 FA eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA), a heavy reliance on this ingredient by the human, pet food, and equine supplement industries is not environmentally sustainable. Instead, sustainable sourcing of plant-based oils rich in n-3 α-linolenic acid (ALA), such as flaxseed and camelina oils, emerges as a viable option to support an optimal n-6:n-3 FA ratio. Moreover, ALA may offer health benefits that extend beyond its role as a precursor for endogenous EPA and DHA production. The following review underlines the metabolism and recommendations of n-6 and n-3 FA for dogs, cats, and horses and the ratio between them in promoting optimal health and inflammation management. Additionally, insights into both marine and plant-based n-3 FA sources will be discussed, along with the commercial practicality of using plant oils rich in ALA for the provision of n-3 FA to companion animals.
In the realm of companion animal nutrition, the balance between the n-6 and n-3 fatty acids (FA) is important. The shared metabolic pathway of these two FA families and the respective signaling molecules produced have implications for the well-being of companion animals such as dogs, cats, and even horses. The n-6:n-3 FA ratio of the diet can directly influence inflammatory responses, disease management, and overall health. Given the prevalent use of n-6 FA-rich vegetable oils in both human and animal diets, there is a growing need to supplement these animals’ diets with n-3 FA. While fish oils containing the long-chain n-3 FA eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been the conventional choice, their overreliance is environmentally unsustainable. Plant-based oils abundant in the n-3 FA α-linolenic acid (ALA) such as flaxseed and camelina oils should be considered, especially given the health benefits of ALA that extend beyond its role as a precursor to EPA and DHA. This review examines the importance of n-3 FA and the n-6:n-3 FA ratio in companion animal diets on animal health while discussing environmentally sustainable alternatives to fish oil to supplement n-3 FA.

BACKGROUND: Rectal temperature (RT) is an important index of core temperature, which has guiding significance for the diagnosis and treatment of pet diseases.
OBJECTIVE: Development and evaluation of an alternative method based on machine learning to determine the core temperatures of cats and dogs using surface temperatures.
METHODS: 200 cats and 200 dogs treated between March 2022 and May 2022.
METHODS: A group of cats and dogs were included in this study. The core temperatures and surface body temperatures were measured. Multiple machine learning methods were trained using a cross-validation approach and evaluated in one retrospective testing set and one prospective testing set.
RESULTS: The machine learning models could achieve promising performance in predicting the core temperatures of cats and dogs using surface temperatures. The root mean square errors (RMSE) were 0.25 and 0.15 for cats and dogs in the retrospective testing set, and 0.15 and 0.14 in the prospective testing set.
CONCLUSIONS: The machine learning model could accurately predict core temperatures for companion animals of cats and dogs using easily obtained body surface temperatures.

Pet acquisition purportedly increased during the COVID-19 pandemic with individuals acquiring pets during periods of social isolation. Families with children experienced unique challenges during the COVID-19 pandemic, balancing childcare, remote schooling, and other needs and therefore patterns of pet acquisition and loss may differ from the broader population. The goal of this study was to understand patterns of pet ownership within families with adolescents during the pandemic to help identify areas for improved support and programmatic recommendations. Using self-reported survey data from a sample of 7,590 American adolescents from the Adolescent Brain Cognitive Development (ABCD) Study® COVID Survey, we found no evidence for large-scale changes in pet acquisition or relinquishment during the first year of the pandemic for families with adolescents in the U.S. Future research should explore the effects of pet acquisition and pet loss on families with adolescents and what resources are needed to support pet ownership during stressors such as the COVID-19 pandemic.

This paper explores what happens to care, and decisions about ending and extending life, when research animals become pets and pets become research animals. To do this, we draw on in-depth qualitative research on (i) rehoming of laboratory animals, (ii) veterinary clinical research, and (iii) the role of the Named Veterinary Surgeon (NVS) in UK animal research. We begin by exploring how (in theory and practice) the ethical, affective, and practical elements of care are split in the research laboratory. We then investigate arguments for and against ending and extending animal life via clinical research and rehoming, highlighting how these activities bring norms and dilemmas around animal death in the laboratory and veterinary clinic to the fore. We conclude by demonstrating the value of investigating borders between animal categories for understanding dilemmas around care and death, and for contributing to emerging literatures within geography around animal care, death, and categorisation. Key contributions of our work include highlighting: how care roles can be split; the importance of considering speculative and in-practice elements of care; the context-dependency and multiplicity of practices of killing in the veterinary clinic and laboratory; and the flexibility and changing nature of animal categories.
Este artículo explora lo que sucede con el cuidado y las decisiones sobre el final y la extensión de la vida cuando los animales de investigación se convierten en mascotas y las mascotas se convierten en animales de investigación. Para hacer esto, nos basamos en una investigación cualitativa a profundidad sobre (i) el realojamiento de animales de laboratorio, (ii) la investigación clínica veterinaria y (iii) el papel del Cirujano Veterinario Designado (CVD o NVS por sus siglas en inglés) en la investigación con animales en el Reino Unido. Comenzamos explorando cómo (en la teoría y la práctica) los elementos éticos, afectivos y prácticos del cuidado se dividen en el laboratorio de investigación. Luego investigamos los argumentos a favor y en contra de terminar y extender la vida animal a través de la investigación clínica y el realojamiento, destacando cómo estas actividades ponen de manifiesto las normas y los dilemas en torno a la muerte animal en el laboratorio y la clínica veterinaria. Concluimos demostrando el valor de investigar las fronteras entre las categorías de animales para comprender los dilemas sobre el cuidado y la muerte, y para contribuir a las literaturas emergentes dentro de la geografía sobre el cuidado, la muerte y la categorización de los animales. Las contribuciones clave de nuestro trabajo incluyen destacar: cómo se pueden dividir los roles de cuidado; la importancia de considerar elementos especulativos y de práctica de la atención; la dependencia del contexto y la multiplicidad de prácticas de matanza en la clínica veterinaria y el laboratorio; y la flexibilidad y naturaleza cambiante de las categorías de animales.
Cet article étudie ce qu’il advient du care et des décisions concernant l’arrêt ou l’extension de la vie, quand les animaux de recherche deviennent animaux de compagnie et que les animaux de compagnie deviennent animaux de recherche. Pour ce faire, nous nous appuyons sur une recherche qualitative approfondie sur (i) l’adoption d’animaux de laboratoire (ii) la recherche clinique vétérinaire, et (iii) le rôle du chirurgien vétérinaire nommé (NVS - Named Veterinary Surgeon) dans la recherche animale au Royaume-Uni. Nous commençons en explorant la manière dont, en théorie et en pratique, les éléments éthiques, affectifs et pratiques du care sont divisés dans le laboratoire de recherche. Nous passons ensuite en revue les arguments pour ou contre l’arrêt ou l’extension de la vie animale par le biais de la recherche clinique et l’adoption, en soulignant la façon dont ces activités amènent au premier plan les normes et les dilemmes autour de la mort animal en laboratoire et dans les cliniques vétérinaires. Nous concluons en démontrant la valeur de l’étude des frontières entre les catégories d’animaux pour comprendre les problèmes entourant le care et la mort, et pour contribuer aux recherches naissantes dans la géographie concernant le care, la mort et la catégorisation des animaux. Les contributions majeures de nos travaux comprennent la mise en évidence de: la manière dont les rôles de care peuvent être divisés; l’importance de la prise en compte d’éléments de care spéculatifs et dans la pratique ; le rapport au contexte et la multiplicité des pratiques de mise à mort dans les cliniques et les laboratoires vétérinaires ; et la flexibilité et la nature changeante des catégories d’animaux.

The bone is a large and complex organ (12-15% of body weight) consisting of specialized connective tissues (bone matrix and bone marrow), whereas joints are composed of cartilage, tendons, ligaments, synovial joint capsules and membranes, and a synovial joint cavity filled with synovial fluid. Maintaining healthy bones and joints is a dynamic and complex process, as bone deposition (formation of new bone materials) and resorption (breakdown of the bone matrix to release calcium and phosphorus) are the continuous processes to determine bone balance. Bones are required for locomotion, protection of internal organs, and have endocrine functions to maintain mineral homeostasis. Joints are responsible for resisting mechanical stress/trauma, aiding in locomotion, and supporting the overall musculoskeletal system. Amino acids have multiple regulatory, compositional, metabolic, and functional roles in maintaining the health of bones and joints. Their disorders are prevalent in mammals and significantly reduce the quality of life. These abnormalities in companion animals, specifically cats and dogs, commonly lead to elective euthanasia due to the poor quality of life. Multiple disorders of bones and joints result from genetic predisposition and are heritable, but other factors such as nutrition, growth rate, trauma, and physical activity affect how the disorder manifests. Treatments for cats and dogs are primarily to slow the progression of these disorders and assist in pain management. Therapeutic supplements such as Cosequin and formulated diets rich in amino acids are used commonly as treatments for companion animals to reduce pain and slow the progression of those diseases. Also, amino acids (e.g., taurine, arginine, glycine, proline, and 4-hydroxyproline), and glucosamine reduce inflammation and pain in animals with bone and joint disorders. Gaining insight into how amino acids function in maintaining bone and joint health can aid in developing preventative diets and therapeutic supplementations of amino acids to improve the quality of life in companion animals.

UNASSIGNED: Companion animals (CAs) may benefit human health, but few studies have examined their impact on stroke survivors. This study examines participation, quality of life (QoL), attachment, cognition, and executive function differences between stroke survivors living with and without CAs.
UNASSIGNED: In this cross-sectional, community-setting study, 25 stroke survivors with a CA and a matched group of 27 without a CA completed standard tools.
UNASSIGNED: Stroke survivors with a CA scored significantly higher in participation and 1 cognitive performance test. No significant differences were found in other cognitive measures or QoL, and attachment to a CA was not correlated with participation or QoL within the research group. However, lower attachment avoidance correlated with better participation in survivors living with dogs.
UNASSIGNED: Living with CAs, especially dogs, might be associated with some cognitive function and participation benefits among stroke survivors. The link between CAs and cognitive function is unclear: Survivors with higher cognitive functioning might be more capable of caring for a CA, or having and caring for a CA might promote better cognitive function. Attachment patterns also might explain stroke survivors\' participation levels. Further study is warranted.