几个世纪以来,Cirsium属的各种物种已在全世界的传统医学中使用。许多民族药理学报告指出,Cirsium植物可以用于减少消化问题。其中,小圆圈(L.)警察。(菊科)是一种有前途的草药候选药物,因为它的成分表现出抗菌和抗氧化的潜力,正如民族药理学报道所证明的那样。因此,该物种作为功能性胃肠和运动障碍的辅助疗法特别有趣。我们的研究目标是验证提取物是如何,分数,C.palustre的主要黄酮在离体条件下影响结肠收缩力。使用猪分离的结肠样本的替代模型来鉴定C.palustre制剂及其主要类黄酮的作用。LC-ESI-MS用于评估甲醇(CP1)的影响,50%甲醇(CP2),和水(CP3)提取物以及乙醚(CP4),乙酸乙酯(CP5),和正丁醇(CP6)馏分。此外,四种类黄酮的影响,芹菜素(API),木犀草素(LUT),芹菜素7-O-葡糖苷酸(A7GLC),和chrysoeriol(CHRY),在等轴测条件下评估了自发和乙酰胆碱诱导的运动性。结果表明,分数,它们的类黄酮对结肠平滑肌表现出有效的运动调节作用。对自发和乙酰胆碱诱导的收缩性观察到运动性调节作用。所有提取物和级分均表现出结肠平滑肌的自发收缩性增强。对于乙酰胆碱诱导的活性,CP1,CP2和CP4引起解痉挛作用,CP5和CP6有痉挛效应。在自发和乙酰胆碱诱导的活性的情况下,LUT和CHRY显示出解痉挛作用。相比之下,API和A7GLC在自发和药理学诱导活性的情况下显示出收缩作用。考虑到从研究中获得的结果,C.palustre可以潜在地提供治疗以动力不足和运动过度为特征的功能性胃肠疾病的益处。
For centuries, various species from the genus Cirsium have been utilized in traditional medicine worldwide. A number of ethnopharmacological reports have pointed out that Cirsium plants can be applied to diminish digestive problems. Among them, Cirsium palustre (L.) Scop. (Asteraceae) stands out as a promising herbal drug candidate because its constituents exhibit antimicrobial and antioxidant potential, as evidenced by ethnopharmacological reports. As a result, the species is particularly intriguing as an adjunctive therapy for functional gastrointestinal and motility disorders. Our research goal was to verify how the extracts, fractions, and main flavonoids of C. palustre affect colon contractility under ex vivo conditions. An alternative model with porcine-isolated colon specimens was used to identify the effects of C. palustre preparations and their primary flavonoids. LC-ESI-MS was utilized to evaluate the impacts of methanol (CP1), methanolic 50% (CP2), and aqueous (CP3) extracts as well as diethyl ether (CP4), ethyl acetate (CP5), and n-butanol (CP6) fractions. Additionally, the impacts of four flavonoids, apigenin (API), luteolin (LUT), apigenin 7-O-glucuronide (A7GLC), and chrysoeriol (CHRY), on spontaneous and acetylcholine-induced motility were assessed under isometric conditions. The results showed that C. palustre extracts, fractions, and their flavonoids exhibit potent motility-regulating effects on colonic smooth muscle. The motility-regulating effect was observed on spontaneous and acetylcholine-induced contractility. All extracts and fractions exhibited an enhancement of the spontaneous contractility of colonic smooth muscle. For acetylcholine-induced activity, CP1, CP2, and CP4 caused a spasmolytic effect, and CP5 and CP6 had a spasmodic effect. LUT and CHRY showed a spasmolytic effect in the case of spontaneous and acetylcholine-induced activity. In contrast, API and A7GLC showed a contractile effect in the case of spontaneous and pharmacologically induced activity. Considering the results obtained from the study, C. palustre could potentially provide benefits in the treatment of functional gastrointestinal disorders characterized by hypomotility and hypermotility.