抗氧化肽是目前食品科学研究的热点,制药,和化妆品。在不同的领域,筛选,活动评估,机制,抗氧化肽的应用是研究的关键领域。在这些主题中,抗氧化肽的有效筛选处于前沿研究的前沿。为此,新技术的高效筛选显著加快了研究进程,逐渐取代传统方法。经过对新型抗氧化肽的筛选和鉴定,耗时的活动评估是另一个不可或缺的过程,尤其是在体内模型中。细胞和啮齿动物模型已广泛用于活动评估,而非啮齿动物模型提供了一个有效的解决方案,即使具有高通量筛选的潜力。同时,分子机制的进一步研究可以阐明活性的本质,这与几个信号通路有关,包括Keap1-Nrf2/ARE,线粒体依赖性凋亡,TGF-β/SMAD,AMPK/SIRT1/PGC-1α,PI3K/Akt/mTOR,和NF-κB。最后但并非最不重要的,抗氧化肽在食品制造中具有广泛的应用,治疗,和化妆品行业,这需要系统的审查。本文综述了抗氧化肽的高效筛选新技术,用一个新的愿景分类。广泛的活性评估测定,包括细胞模型,以及啮齿动物和非啮齿动物模型,以全面的方式提供。此外,结合具体案例分析了分子机制的最新进展。最后,抗氧化肽在食品生产中的应用,治疗,并对化妆品进行了系统综述。
Antioxidant peptides are currently a hotspot in food science, pharmaceuticals, and cosmetics. In different fields, the screening, activity evaluation, mechanisms, and applications of antioxidant peptides are the pivotal areas of research. Among these topics, the efficient screening of antioxidant peptides stands at the forefront of cutting-edge research. To this end, efficient screening with novel technologies has significantly accelerated the research process, gradually replacing the traditional approach. After the novel antioxidant peptides are screened and identified, a time-consuming activity evaluation is another indispensable procedure, especially in in vivo models. Cellular and rodent models have been widely used for activity evaluation, whilst non-rodent models provide an efficient solution, even with the potential for high-throughput screening. Meanwhile, further research of molecular mechanisms can elucidate the essence underlying the activity, which is related to several signaling pathways, including Keap1-Nrf2/ARE, mitochondria-dependent apoptosis, TGF-β/SMAD, AMPK/SIRT1/PGC-1α, PI3K/Akt/mTOR, and NF-κB. Last but not least, antioxidant peptides have broad applications in food manufacture, therapy, and the cosmetics industry, which requires a systematic review. This review introduces novel technologies for the efficient screening of antioxidant peptides, categorized with a new vision. A wide range of activity evaluation assays, encompassing cellular models, as well as rodent and non-rodent models, are provided in a comprehensive manner. In addition, recent advances in molecular mechanisms are analyzed with specific cases. Finally, the applications of antioxidant peptides in food production, therapy, and cosmetics are systematically reviewed.