■肺炎克雷伯菌分离株的抗生素耐药性,特别是对粘菌素的抗性,已经成为一个越来越令人担忧的问题。本研究旨在调查特定基因(pmrA,pmrB,pmrC,phoQ,phoP,和arnT),这有助于从德黑兰的人类临床样本中收集的肺炎克雷伯菌分离株的粘菌素耐药性,伊朗。
■获得了38株肺炎克雷伯菌分离株,并进行了抗生素药敏试验,以及根据CLSI指南评估表型AmpC和ESBL的产生。使用聚合酶链反应(PCR)进行抗生素抗性基因的调查,而粘菌素抗性相关基因表达的定量是通过实时PCR进行的。
■观察到头孢噻肟33(86.8%)和米诺环素8(21.1%)的抗生素耐药性最高和最低,分别。24株(63.2%)和31株(81.6%)分离株携带AmpC和ESBLs,分别。此外,含有blaNDM的抗生素抗性基因,blaIMP,BlaVIM,blaSHV,blaTEM,blaCTXM,qnrA,qnrB,qnrS,在肺炎克雷伯菌分离株中检测到aac(6')-Ib。只有5个(13.1%)分离株对粘菌素具有抗性,这些分离株的MIC范围在4至64μgml-1之间。pmrA的上调,pmrB,pmrC,phoQ,phoP,在粘菌素抗性分离株中观察到arnT基因。发现粘菌素抗性分离株同时存在ESBLs,AMPC,氟喹诺酮,氨基糖苷类,和碳青霉烯类耐药基因。
■这项研究揭示了肺炎克雷伯菌的抗生素耐药性不断升级,各种抗性性状显著共存,强调需要警惕的监测和创新的干预措施。
UNASSIGNED: Antibiotic resistance in Klebsiella pneumoniae isolates, particularly resistance to colistin, has become a growing concern. This study seeks to investigate the upregulation of specific genes (pmrA, pmrB, pmrC, phoQ, phoP, and arnT) that contribute to colistin resistance in K. pneumoniae isolates collected from human clinical samples in Tehran, Iran.
UNASSIGNED: Thirty eight K. pneumoniae isolates were obtained and subjected to antibiotic susceptibility testing, as well as evaluation for phenotypic AmpC and ESBL production according to CLSI guidelines. The investigation of antibiotic resistance genes was conducted using polymerase chain reaction (PCR), whereas the quantification of colistin resistance related genes expressions was performed via Real-Time PCR.
UNASSIGNED: The highest and lowest antibiotics resistance were observed for cefotaxime 33 (86.8%) and minocycline 8 (21.1%), respectively. Twenty-four (63.2%) and 31 (81.6%) isolates carried AmpC and ESBLs, respectively. Also, antibiotic resistance genes containing blaNDM, blaIMP, blaVIM, blaSHV, blaTEM, blaCTXM, qnrA, qnrB, qnrS, and aac(6\')-Ib were detected in K. pneumoniae isolates. Only 5 (13.1%) isolates were resistant to colistin and the MIC range of these isolates was between 4 and 64 μg ml-1. Upregulation of the pmrA, pmrB, pmrC, phoQ, phoP, and arnT genes was observed in colistin-resistant isolates. The colistin-resistant isolates were found to possess a simultaneous presence of ESBLs, AmpC, fluoroquinolone, aminoglycoside, and carbapenem resistant genes.
UNASSIGNED: This study reveals escalating antibiotic resistance in K. pneumoniae, with notable coexistence of various resistance traits, emphasizing the need for vigilant surveillance and innovative interventions.