吡虫啉的应用,作为油菜上的种子包衣剂,建议控制芥末蚜虫,Lipphiserysimi(Kaltenbach)(半翅目:蚜科)。在这项研究中,研究了L.erysimi的反应,包括生存,喂养行为,解毒酶,在由吡虫啉包衣的种子以6、12或18g活性成分(a.i.)/kg种子的速率生长的油菜幼苗上。结果表明,蚜虫的存活率,连同幸存者的后代,在种子处理的幼苗上明显减少。这表明可以有效抑制田间蚜虫种群。电渗透图(EPG)技术用于记录蚜虫对两个,four-,和用吡虫啉包衣的油菜幼苗的六叶阶段,在蚜虫的摄食行为过程中发现了个体反应。在两叶期的植物上,蚜虫的摄食行为受到影响,显示探针渗透到叶片(探针)或叶肉细胞中的频率降低(潜在的下降,简称pds),并缩短了叶片(探针)或韧皮部中探针事件的持续时间。在四叶和六叶阶段的植物上,吡虫啉的这些影响被削弱了;然而,在所有吡虫啉治疗中,韧皮部的唾液分泌持续时间缩短至小于5分钟。在用吡虫啉处理的幼苗上维持的蚜虫中混合功能氧化酶的活性升高。总之,吡虫啉可用作防治蚜虫的种衣剂,但是蚜虫的耐化学性不容忽视。
Imidacloprid application, as a seed coating agent on oilseed rape, is recommended to control mustard aphid, Lipaphis erysimi (Kaltenbach) (Hemiptera: Aphididae). In this study, responses of L. erysimi were investigated, including survival, feeding behavior, and detoxifying enzymes, on the oilseed rape seedlings grown from seeds coated with imidacloprid at rates of 6, 12, or 18 g active ingredient (a.i.)/kg seed. The results showed that the aphids\' survival rate, together with that of the progeny of the survivors, on the seed-treated seedlings significantly decreased. This indicates that the aphid population in fields can be suppressed effectively. The electrical penetration graph (EPG) technique was used to record aphid feeding behaviors on two-, four-, and six-leaf stages of oilseed rape seedlings that had been seed-coated with imidacloprid, and individual responses were revealed during the aphid feeding behavior. On the plants at the two-leaf stage, aphid feeding behaviors were influenced, showing decreased frequency of stylet penetration into the leaf (probe) or into the mesophyll cells (potential drops, pds for short), and shortened duration of stylet event in the leaf (probe) or in the phloem. On the plants at the four- and six-leaf stages, these impacts of imidacloprid were weakened; however, the saliva secretion duration in phloem was shortened to less than 5 min in all imidacloprid treatments. The activity of mixed-function oxidase in aphids maintained on the treated seedlings with imidacloprid was elevated. In conclusion, imidacloprid could be used as a seed coating agent for aphid control, but chemical resistance in aphids should not be ignored.