耐甲氧西林金黄色葡萄球菌(MRSA)是公共卫生领域关注的重要原因。食物链在该病原体传播和抗菌素耐药性(AMR)中的作用尚未确定。这项工作的目的是分离和表征凝固酶阳性葡萄球菌(CoPS)和凝固酶阴性葡萄球菌(CoNS),特别是金黄色葡萄球菌,从位于阿根廷的学校餐厅。从处理程序获得的95个样本中,惰性表面,食物,和10个机构的空气,分离出30株葡萄球菌。四个分离株是金黄色葡萄球菌,其余(N=26)属于11种凝固酶阴性物种(CoNS)。测试了分离株对9种抗生素的敏感性。存在编码毒素的基因(luk-PV,海,seb,sec,SED,andsee),粘附素(icaA,icaD),并研究了赋予耐甲氧西林(mecA)和万古霉素(vanA)抗性的基因。测量的青霉素耐药率,头孢西丁,庆大霉素,万古霉素,红霉素,克林霉素,左氧氟沙星,甲氧苄啶-磺胺甲恶唑,四环素占73%,30%,13%,3%,33%,17%,13%,7%,7%的分离株,分别。检测到17个AMR配置文件,11株多重耐药(MDR)。在来自四个机构的处理人员手中检测到七个耐甲氧西林葡萄球菌分离株,其中两个是MRSA。两个金黄色葡萄球菌分离株呈现icaA和icaD,另一个,只有icaD。在两个分离株中发现了vanA基因。关于金黄色葡萄球菌,检测到对万古霉素的耐药性,但对庆大霉素没有耐药性。学校供餐在儿童营养中起着关键作用,食用受MRSA和耐万古霉素金黄色葡萄球菌(VRSA)污染的食物可能对健康构成严重威胁。特别是,检测到处理人员是MRSA的来源,VRSA,MR-CoNS(耐甲氧西林凝固酶阴性葡萄球菌),和MDR分离株。获得的结果表明,学校餐厅对这种病原体的警惕性应该是极端的。
Methicillin-resistant Staphylococcus aureus (MRSA) constitutes an important cause for concern in the field of public health, and the role of the food chain in the transmission of this pathogen and in antimicrobial resistance (AMR) has not yet been defined. The objectives of this work were to isolate and characterize coagulase-positive Staphylococcus (CoPS) and coagulase-negative Staphylococcus (CoNS), particularly S. aureus, from school dining rooms located in Argentina. From 95 samples that were obtained from handlers, inert surfaces, food, and air in 10 establishments, 30 Staphylococcus strains were isolated. Four isolates were S. aureus, and the remaining ones (N = 26) belonged to 11 coagulase-negative species (CoNS). The isolates were tested for susceptibility to nine antibiotics. The presence of genes encoding toxins (luk-PV, sea, seb, sec, sed, and see), adhesins (icaA, icaD), and genes that confer resistance to methicillin (mecA) and vancomycin (vanA) was investigated. The resistance rates measured for penicillin, cefoxitin, gentamicin, vancomycin, erythromycin, clindamycin, levofloxacin, trimethoprim-sulfamethoxazole, and tetracycline were 73%, 30%, 13%, 3%, 33%, 17%, 13%, 7%, and 7% of the isolates, respectively. Seventeen AMR profiles were detected, and 11 isolates were multidrug resistant (MDR). Seven methicillin-resistant Staphylococcus isolates were detected in the hands of handlers from four establishments, two of them were MRSA. Two S. aureus isolates presented icaA and icaD, another one, only icaD. The gene vanA was found in two isolates. In relation to S. aureus, resistance to vancomycin but not to gentamicin was detected. School feeding plays a key role in the nutrition of children, and the consumption of food contaminated with MRSA and vancomycin-resistant S. aureus (VRSA) can be a serious threat to health. In particular, it was detected that the handlers were the source of MRSA, VRSA, MR-CoNS (methicillin-resistant coagulase-negative Staphylococcus), and MDR isolates. The results obtained indicate that the vigilance of this pathogen in school dining rooms should be extreme.