■植物性饲料添加剂是源自植物的产品,用于改善动物的性能和健康。如今,这种植物性饲料添加剂被广泛用作家禽饲料中抗生素生长促进剂的替代品,丁香芽是引起研究人员注意的最有效的草药之一。
■本实验旨在评估在肉鸡饲料中添加丁香芽粉对血液学特征的影响,生化参数,淋巴器官,和细胞介导的免疫。
■总共360只无性别的小鸡(Ross308)被分配到三组。所有小鸡在相同的正常管理条件下从1天至6周龄饲养。第一组饲喂基础日粮,其他两组在基础日粮中添加0.5%和1.0%的丁香芽粉。研究的性状是白细胞总数(TLC),分类白细胞计数(DLC),血胆固醇水平,血肝酶[天冬氨酸转氨酶,丙氨酸氨基转移酶,和碱性磷酸酶(ALP)酶],淋巴器官(法氏囊和脾脏)的重量,和细胞介导的免疫。
■尽管在第二和第三周向肉鸡饲料中添加丁香芽粉末导致了显着的变化(p≤0.05),实验结束时的结果表明,添加丁香粉对TLC和DLC没有负面影响,以及嗜红细胞/淋巴细胞的比例。此外,以含有丁香芽粉的饮食为食对血液中的胆固醇和肝酶水平没有显着影响,除了(ALP),与对照组相比,显着增加(p≤0.01)。此外,结果显示在第二和第五周显著增加(p≤0.05)的相对重量的脾脏,但是,总的来说,在实验结束时,对淋巴器官的相对重量没有显着影响。此外,以0.5%的比例食用丁香芽粉的肉鸡表现出高度显着的(p≤0.01)细胞免疫反应。
■该研究得出结论,丁香芽粉的添加对白细胞计数或分化白细胞计数没有负面影响。该添加还提高了肉鸡在生长阶段的脾脏重量并提高了血液烷烃磷酸酶活性和细胞免疫应答水平。
Phytogenic feed additives are products derived from plants used to improve the performance and health of animals. Nowadays, this type of phytogenic feed additive is widely used as an alternative to antibiotic growth promoters in poultry feed, and
clove bud is one of the most effective medicinal herbs that has caught the attention of researchers.
This experiment was conducted to evaluate the effects of adding
clove bud powder to the broiler chicken\'s feed on the hematological profile, biochemical parameters, lymphoid organs, and cell-mediated immunity.
A total of 360 unsexed chicks (Ross 308) were allotted to three groups. All chicks were raised under the same normal management conditions from 1 day to 6 weeks of age. The first group was fed a basal diet and the other two groups were assigned to add 0.5% and 1.0% of
clove bud powder to the basal diet. The studied traits were total leukocyte counts (TLC), differential leukocyte counts (DLC), blood cholesterol level, blood liver enzymes [aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase (ALP) enzymes], the weight of lymphoid organs (bursa of Fabricius and spleen), and cell-mediated immunity.
Despite the significant and varied changes (p ≤ 0.05) that resulted from adding
clove buds powder to the broiler feed in the second and third weeks, the results at the end of the experiment indicated that there was no negative effect of adding clove powder on the TLC and DLC, as well as the heterophils/lymphocytes ratio. In addition, feeding on a diet containing
clove buds powder had no significant effect on the level of cholesterol and liver enzymes in the blood, except for (ALP), which showed a significant increase (p ≤ 0.01) in comparison to the control group. Moreover, the results showed in the second and fifth weeks a significant increase (p ≤ 0.05) in the relative weight of the spleen, but, in general, there were no significant effects at the end of the experiment on the relative weight of the lymphoid organs. Furthermore, the broiler chickens that consumed clove bud powder at a rate of 0.5% showed a highly significant (p ≤ 0.01) cellular immune response.
This study concluded that the addition of clove bud powder had no negative effect on leukocyte counts or differentiated leukocyte counts. The addition also raised the spleen weight and improved the level of blood alkane phosphatase activity and cellular immune response in broiler chickens during the growth stages.