有丝分裂保真度对于将遗传信息忠实地分布到子细胞中至关重要。许多真菌物种,包括裂殖酵母,经历闭合形式的有丝分裂,在此期间,核外壳不会破裂。在S.Pombe,已经确定了许多有助于成功完成有丝分裂的过程。值得注意的是,脂质代谢的扰动可导致灾难性的有丝分裂和“切割”表型。有人认为,这些有丝分裂缺陷是由后期核扩增过程中膜磷脂供应不足引起的。然而,目前尚不清楚是否涉及其他因素。在这项研究中,我们详细表征了缺乏Cbf11转录因子的S.pombe突变体的有丝分裂,调节脂质代谢基因。我们表明,在cbf11Δ细胞中,有丝分裂缺陷在后期之前已经出现,在核扩张开始之前。此外,我们确定了改变的凝聚素动力学和着丝粒染色质结构,作为影响脂质稳态破坏的细胞有丝分裂保真度的其他因素,提供对这一基本生物学过程的新见解。
Mitotic fidelity is crucial for the faithful distribution of genetic information into the daughter cells. Many fungal species, including the fission yeast Schizosaccharomyces pombe, undergo a closed form of mitosis, during which the nuclear envelope does not break down. In S. pombe, numerous processes have been identified that contribute to successful completion of mitosis. Notably, perturbations of lipid metabolism can lead to catastrophic mitosis and the \'cut\' phenotype. It has been suggested that these mitotic defects are caused by insufficient membrane phospholipid supply during the anaphase nuclear expansion. However, it is not clear whether additional factors are involved. In this study, we characterized in detail mitosis in an S. pombe mutant lacking the Cbf11 transcription factor, which regulates lipid metabolism genes. We show that in cbf11Δ cells mitotic defects have already appeared prior to anaphase, before the nuclear expansion begins. Moreover, we identify altered cohesin dynamics and centromeric chromatin structure as additional factors affecting mitotic fidelity in cells with disrupted lipid homeostasis, providing new insights into this fundamental biological process.