植物广泛用于传统的医学系统,其中许多会对生殖系统产生不利影响。cleistanthuscollinus是一种含有许多活性植物化学物质的植物,有可能被开发为药物。本研究旨在评估C.collinus的水提取物对男性生殖系统的影响。
雄性Wistar大鼠用不同剂量的C.collinus(200和400mg/kg,口头),或生理盐水28天,并评估其对生殖系统的影响。环磷酰胺(100mg/kg,每周,i.p),一种著名的生殖毒物,用作阳性对照。
C.collinus治疗后,精子数量和运动能力显著降低,随着初级精母细胞的破坏,精子细胞和基底层厚度减小。LH,FSH和睾酮水平显著降低。生精管面积的减少表明生殖细胞和支持细胞的破坏。C.collinus处理增加了氧化应激,丙二醛水平升高以及过氧化氢酶和GSH活性降低证明了这一点。BAX的表达,在精母细胞中观察到BCL-2和p53,表明细胞凋亡增加。
C.collinus可以产生雄性生殖毒性,这可能是通过荷尔蒙水平的改变来介导的,进而干扰精子发生。它可能会增加睾丸中凋亡因子的表达并消耗保护性抗氧化酶。
Plants are widely used in the traditional system of medicine and many of them can adversely affect the reproductive system. Cleistanthus collinus is a plant containing many active phytochemicals, which have the potential to be developed as a drug. The present study was aimed to evaluate the effects of an aqueous extract of C. collinus on the male reproductive system.
Male Wistar rats were treated with different doses of C. collinus (200 and 400 mg/kg, orally), or with saline for 28 days and its effect on the reproductive system was assessed. Cyclophosphamide (100 mg/kg, weekly, i.p), a well-known reproductive toxicant, was used as a positive control.
There was a significant reduction in sperm count and motility with C. collinus treatment, along with the destruction of primary spermatocytes, spermatids and reduced thickness of the basal layer. The LH, FSH and testosterone levels were reduced significantly. A reduction in the area of seminiferous tubules indicates the destruction of germ cells and Sertoli cells. C. collinus treatment increased the oxidative stress, evidenced by elevated malondialdehyde levels along with a reduction in catalase and GSH activities. The expression of BAX, BCL-2 and p53 was observed in spermatocytes, indicating an increase in apoptosis.
C. collinus can produce male reproductive toxicity, which may be mediated through alterations in hormonal levels, which in turn interferes with spermatogenesis. It may increase the expression of apoptotic factors and deplete protective antioxidant enzymes in the testis.