我们的目的是研究内皮功能障碍生物标志物与肝硬化表现的关联,细菌易位,和肠道微生物群。使用16SrRNA基因测序评估粪便微生物组。血浆亚硝酸盐水平,大内皮素-1,不对称二甲基精氨酸(ADMA),presepsin,claudin被测量为内皮功能障碍的生物标志物,细菌易位,和肠屏障功能障碍。进行了同时测定血压和心率的超声心动图以评估血液动力学参数。Presepsin,claudin3,亚硝酸盐,肝硬化患者的ADMA水平高于对照组。升高的亚硝酸盐水平与高水平的presepsin和claudin3,血流动力学循环的发展有关,低蛋白血症,2-3级腹水,明显的肝性脑病,高平均肺动脉压,变形杆菌和丹毒杆菌的丰度增加,和减少的数量,下颗粒,Rikenellaceae,酸性球菌科,Christensenellaceae,和厌氧科。ADMA水平升高与Child-Pugh评分较高相关,降低血清钠水平,低蛋白血症,2-3级腹水,轻度食管静脉曲张,明显的肝性脑病,较低的平均肺动脉压,和低丰度的丹毒科和丹毒科。高水平的内皮素-1与高水平的presepsin和钠有关,低水平的纤维蛋白原和胆固醇,低凝,增加了嗜双菌和芽孢杆菌的丰度,并降低了Alloprevotella的丰度。
Our aim was to study the association of endothelial dysfunction biomarkers with cirrhosis manifestations, bacterial translocation, and gut microbiota taxa. The fecal microbiome was assessed using 16S rRNA gene sequencing. Plasma levels of nitrite, big endothelin-1, asymmetric dimethylarginine (ADMA), presepsin, and claudin were measured as biomarkers of endothelial dysfunction, bacterial translocation, and intestinal barrier dysfunction. An echocardiography with simultaneous determination of blood pressure and heart rate was performed to evaluate hemodynamic parameters. Presepsin, claudin 3, nitrite, and ADMA levels were higher in cirrhosis patients than in controls. Elevated nitrite levels were associated with high levels of presepsin and claudin 3, the development of hemodynamic circulation, hypoalbuminemia, grade 2-3 ascites, overt hepatic encephalopathy, high mean pulmonary artery pressure, increased abundance of Proteobacteria and Erysipelatoclostridium, and decreased abundance of Oscillospiraceae, Subdoligranulum, Rikenellaceae, Acidaminococcaceae, Christensenellaceae, and Anaerovoracaceae. Elevated ADMA levels were associated with higher Child-Pugh scores, lower serum sodium levels, hypoalbuminemia, grade 2-3 ascites, milder esophageal varices, overt hepatic encephalopathy, lower mean pulmonary artery pressure, and low abundance of Erysipelotrichia and Erysipelatoclostridiaceae. High big endothelin-1 levels were associated with high levels of presepsin and sodium, low levels of fibrinogen and cholesterol, hypocoagulation, increased Bilophila and Coprobacillus abundances, and decreased Alloprevotella abundance.