吲哚金杆菌和回肠金杆菌是革兰氏阴性环境细菌,经常被报道与致命的医院感染有关。在过去的几十年中,免疫功能低下的个体出现了菌血症和呼吸机相关性肺炎。金黄杆菌属之间的相互作用。和卡斯特拉尼棘阿米巴,一种在环境中无处不在的自由生活的变形虫,以前没有探索过。在这项研究中,将吲哚梭菌和C.gleum与A.castellanii滋养体共培养,并评估了它们的相互作用。我们的结果表明,当与卡氏A.castellanii共培养时,吲哚梭菌和回肠梭菌的细菌数量显着增加(p<0.05),表明A.castellanii的生长支持作用。具体来说,我们的发现表明,C.indologenes和C.gleum能够联系起来,被A.castellani滋养体入侵和/或吸收,并以相似的速率在细胞内繁殖(p>0.05)。有趣的是,两种金黄杆菌属。相关的,castellanii以明显高于大肠杆菌K1的速率入侵和/或吸收,大肠杆菌K1是一种已知在castellanii细胞内相互作用和复制的神经致病性细菌菌株(p<0.05)。然而,两种金杆菌属的能力。在A.castellanii中繁殖明显弱于大肠杆菌K1(p<0.001)。这是Chryseobacteriumspp。和A.castellanii被证明是相互作用的。在A.castellanii中在细胞内存活的能力可能会赋予对吲哚梭菌和C.gleum的保护,并有助于金杆菌属的存活和传播。医院环境中的易感宿主。未来的研究将确定C.indologenes和C.gleum在A.castellanii囊肿中的存活能力以及参与这种相互作用的可能的分子机制。
Chryseobacterium indologenes and Chryseobacterium gleum are Gram negative environmental bacteria that have been frequently reported to implicate in fatal nosocomial infections, such as bacteraemia and ventilator-associated pneumonia in immunocompromised individuals in the past decades. The interaction between Chryseobacterium spp. and Acanthamoeba castellanii, a free-living amoeba ubiquitous in the environment, has not been explored previously. In this study, C. indologenes and C. gleum were co-cultured with A. castellanii trophozoites and their interactions were evaluated. Our results showed that when co-cultured with A. castellanii, bacterial numbers of C. indologenes and C. gleum increased significantly (p < 0.05), indicating growth-supporting role of A. castellanii. Specifically, our findings showed that C. indologenes and C. gleum were able to associate, invade and/or taken up by A. castellani trophozoites, and multiply intracellularly at similar rates (p > 0.05). Interestingly, the two Chryseobacterium spp. associated, invaded and/or taken up by A. castellanii at significantly higher rates than Escherichia coli K1, a neuropathogenic bacterial strain known to interact and replicate intracellularly in A. castellanii (p < 0.05). However, the ability of both Chryseobacterium spp. to multiply in A. castellanii was significantly weaker than E. coli K1 (p < 0.001). This is the first time that Chryseobacterium spp. and A. castellanii were shown to interact with each other. The ability to survive intracellularly in A. castellanii may confer protection to C. indologenes and C. gleum and assist in the survival and transmission of Chryseobacterium spp. to susceptible hosts within a hospital setting. Future studies will determine the ability of C. indologenes and C. gleum survival in A. castellanii cysts and the possible molecular mechanisms involved in such interactions.