目的:本报告的目的是分析从15号环状染色体的不育男性患者获得的精子的染色体状态和受精能力。
方法:这是一个私人体外受精诊所的病例报告。一名男子被诊断患有严重的少精子症,携带15号环状染色体。为了评估染色体状态和受精能力,将携带15号环状染色体的患者的精子注射到去核的小鼠卵母细胞中。
结果:携带15号环状染色体的患者的活动精子核型正常,在1PN的染色体散布样本中未不雅察到环染色体15。此外,这些能动的精子保留了受精能力。然而,施肥率下降(85.2、76.2和64.3%,分别)随着精子头部长宽比的下降(分别≥1.50、1.30-1.49和<1.30)。
结论:核型正常,没有15号环状染色体,高宽比的活动精子显示出足够的受精潜力。
OBJECTIVE: The purpose of this report is to analyze the chromosome status and fertilization capability of sperm obtained from an infertile male patient with ring chromosome 15.
METHODS: This was a case report at a private in vitro fertilization clinic. A man diagnosed with severe oligozoospermia carrying ring chromosome 15. To evaluate the chromosome status and fertilization capability, sperm from a patient carrying ring chromosome 15 were injected into enucleated mouse oocytes.
RESULTS: The karyotypes of motile sperm from a patient carrying ring chromosome 15 were normal, and ring chromosome 15 was not observed in the chromosome spread samples of 1PN. In addition, these motile sperm retained the fertilization capability. However, the fertilization rates decreased (85.2, 76.2, and 64.3%, respectively) along with the decline of the aspect ratio of the sperm head (≥ 1.50, 1.30-1.49, and < 1.30, respectively).
CONCLUSIONS: The karyotypes were normal without ring chromosome 15, and motile sperm with a high aspect ratio showed adequate potential for fertilization.