功能上不同的视网膜神经节细胞在小鼠视网膜上具有密度和大小梯度,一些退行性眼病遵循细胞死亡的地形图特异性梯度。因此,视网膜相对于眼眶和头部的解剖方向对于了解健康和疾病中视网膜的功能解剖非常重要。然而,不同的研究小组使用不同的解剖标志来确定视网膜方向(背侧,腹侧,temporal,鼻杆)。在解剖过程中标记这些标志的准确性和可靠性的变化可能会导致视网膜形貌的识别和报告差异。这项研究的目的是比较眼角的准确性和可靠性,直肌,和脉络膜裂标志在报告视网膜方向。根据其与视蛋白过渡区(OTZ)的关系,计算了在解剖过程中从每个界标获得的视网膜缓解切角。通过自定义MATLAB脚本确定,该脚本将免疫染色的s-视蛋白与视网膜对齐。脉络膜裂和直肌标志是最准确和可靠的,而使用眼角作为参考的烧伤痕迹最少。这些值被用来建立一个解剖地图,绘制各种眼睛标志的相互关系,水平的半规管,到lambda-bregma,和地球的地平线。令人惊讶的是,在正常运动期间,鼠标的视蛋白梯度和水平的半规管使等效的6°角对准地球地平线附近的OTZ,该功能可以增强鼠标在其环境中视觉导航的能力。
Functionally distinct retinal ganglion cells have density and size gradients across the mouse retina, and some degenerative eye diseases follow topographic-specific gradients of cell death. Hence, the anatomical orientation of the retina with respect to the orbit and head is important for understanding the functional anatomy of the retina in both health and disease. However, different research groups use different anatomical landmarks to determine retinal orientation (dorsal, ventral, temporal, nasal poles). Variations in the accuracy and reliability in marking these landmarks during dissection may lead to discrepancies in the identification and reporting of retinal topography. The goal of this study was to compare the accuracy and reliability of the canthus, rectus muscle, and choroid fissure landmarks in reporting retinal orientation. The retinal relieving cut angle made from each landmark during dissection was calculated based on its relationship to the opsin transition zone (OTZ), determined via a custom MATLAB script that aligns retinas from immunostained s-opsin. The choroid fissure and rectus muscle landmarks were the most accurate and reliable, while burn marks using the canthus as a reference were the least. These values were used to build an anatomical map that plots various ocular landmarks in relationship to one another, to the horizontal semicircular canals, to lambda-bregma, and to the earth\'s horizon. Surprisingly, during normal locomotion, the mouse\'s opsin gradient and the horizontal semicircular canals make equivalent 6° angles aligning the OTZ near the earth\'s horizon, a feature which may enhance the mouse\'s ability to visually navigate through its environment.