背景:研究调查了姜黄素和多奈哌齐共同给药对认知功能的几种标志物的影响(例如空间记忆,星形胶质细胞激活,胆碱酯酶的表达)在东pol碱治疗的大鼠的大脑皮层和海马中。
结果:连续7天,给予姜黄素(50mg/kg)和/或多奈哌齐(2.5mg/kg)预处理.第七天,服用东pol碱(1mg/kg)引起认知障碍,在进行记忆测试前30分钟。接下来是评估空间记忆的变化,胆碱酯酶,和腺苷脱氨酶(ADA)活性,以及一氧化氮(NO)水平测定。此外,在大脑皮层和海马中进行了胶质纤维酸性蛋白(GFAP)和胆碱酯酶基因表达的RT-qPCR。此外,在大脑皮层和海马中进行脑组织的GFAP免疫组织化学以进行神经元损伤。与对照组相比,给予东pol碱的大鼠记忆受损,更高水平的乙酰胆碱酯酶(AChE),丁酰胆碱酯酶(BChE),和ADA活动,以及升高的氧化应激标志物。除了增强GFAP免疫反应性,脑组织中也存在GFAP和BChE基因的过表达。姜黄素和多奈哌齐的组合是,然而,与给予多奈哌齐的大鼠组相比,观察到更好地改善了这些损伤。
结论:因此,该证据提供了更多的机制来支持以下假设:姜黄素和多奈哌齐的同时给药减轻了东莨菪碱治疗的大鼠模型中的认知功能障碍标志物.
BACKGROUND: The study investigated the effect of co-administration of curcumin and donepezil on several markers of cognitive function (such as spatial memory, astrocyte activation, cholinesterase expressions) in the brain cortex and hippocampus of scopolamine-treated rats.
RESULTS: For seven consecutive days, a pre-treatment of curcumin (50 mg/kg) and/or donepezil (2.5 mg/kg) was administered. On the seventh day, scopolamine (1 mg/kg) was administered to elicit cognitive impairment, 30 min before memory test was conducted. This was followed by evaluating changes in spatial memory, cholinesterase, and adenosine deaminase (ADA) activities, as well as nitric oxide (NO) level were determined. Additionally, RT-qPCR for glial fibrillary acidic protein (GFAP) and cholinesterase gene expressions was performed in the brain cortex and hippocampus. Also, GFAP immunohistochemistry of the brain tissues for neuronal injury were performed in the brain cortex and hippocampus. In comparison to the control group, rats given scopolamine had impaired memory, higher levels of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and ADA activities, as well as elevated markers of oxidative stress. In addition to enhanced GFAP immunoreactivity, there was also overexpression of the GFAP and BChE genes in the brain tissues. The combination of curcumin and donepezil was, however, observed to better ameliorate these impairments in comparison to the donepezil-administered rat group.
CONCLUSIONS: Hence, this evidence provides more mechanisms to support the hypothesis that the concurrent administration of curcumin and donepezil mitigates markers of cognitive dysfunction in scopolamine-treated rat model.