作为动物的近亲,鞭毛虫提供了对动物细胞生理学起源的见解。这里,我们报告了来自MonoLake的殖民地鞭毛虫的隔离和表征,加州鞭毛虫形成大的球形菌落,比密切相关的鞭毛虫Salpingoecarosetta形成的菌落大一个数量级。在实验室维持的培养物中,球形菌落的内腔充满了细胞外基质的分支网络,并被细菌定植,包括不同的γ变形杆菌和α变形杆菌。我们建议竖立Barroecamonosierragen。11月。,sp.11月。Hake,Burkhardt,Richter,和国王来容纳这种极端微生物鞭毛虫。培养中细菌与B.monosierra之间的物理关联为研究细菌与真核生物之间的相互作用提供了新的实验模型。未来的工作将研究野生种群中这些相互作用的性质以及细菌对B.monosierra球体定植的机制。
目标:生活在莫诺湖极端环境中的生物的多样性(加利福尼亚州,美国)是有限的。我们试图调查动物的近亲,鞭毛虫,存在于莫诺湖,一种高盐,碱性,富砷环境。我们反复分离了一种新型鞭毛虫的成员,我们将其命名为Barroecamonosierra。B.monosierra的表征表明,它形成含有不同的共同分离细菌的大球形菌落,为研究真核生物和细菌之间物理关联的潜在机制提供了机会。
As the closest living relatives of animals, choanoflagellates offer insights into the ancestry of animal cell physiology. Here, we report the isolation and characterization of a colonial choanoflagellate from Mono Lake, California. The choanoflagellate forms large spherical colonies that are an order of magnitude larger than those formed by the closely related choanoflagellate Salpingoeca rosetta. In cultures maintained in the laboratory, the lumen of the spherical colony is filled with a branched network of extracellular matrix and colonized by bacteria, including diverse Gammaproteobacteria and Alphaproteobacteria. We propose to erect Barroeca monosierra gen. nov., sp. nov. Hake, Burkhardt, Richter, and King to accommodate this extremophile choanoflagellate. The physical association between bacteria and B. monosierra in culture presents a new experimental model for investigating interactions among bacteria and eukaryotes. Future work will investigate the nature of these interactions in wild populations and the mechanisms underpinning the colonization of B. monosierra spheres by bacteria.
OBJECTIVE: The diversity of organisms that live in the extreme environment of Mono Lake (California, USA) is limited. We sought to investigate whether the closest living relatives of animals, the choanoflagellates, exist in Mono Lake, a hypersaline, alkaline, arsenic-rich environment. We repeatedly isolated members of a new species of choanoflagellate, which we have named Barroeca monosierra. Characterization of B. monosierra revealed that it forms large spherical colonies containing diverse co-isolated bacteria, providing an opportunity to investigate mechanisms underlying physical associations between eukaryotes and bacteria.