背景:毒死蜱属于广谱有机磷杀虫剂,具有高毒性,通过氧化反应在肝脏中代谢,并能抑制乙酰胆碱酯酶活性。乙酰胆碱酯酶抑制产生活性氧并诱导氧化应激,最终导致像肾脏一样的细胞损伤。检查血尿素氮(BUN)水平,肌酐,肾组织病理学是评估毒死蜱对细胞和肾组织损害程度的适当指标。
方法:这项用于确定毒死蜱暴露持续时间和剂量反应关系的研究对于早期检测毒死蜱毒性对健康的影响很重要。该研究是一项真正的实验(完全随机设计),由30名受试者分为5组。对照组(K1)给予1mg/kgBW吐温20和NaCl0,9%,直至第56天。毒死蜱暴露组(P1,P2,P3和P4)给予毒死蜱5mg/kgBW,持续7、14、28和56天。治疗后,测量BUN和肌酐水平,并分析了肾脏的微观变化。BUN的结果,肌酐,和肾脏组织病理学分析采用方差分析统计检验。
结果:数据结果显示,与对照组相比,BUN和肌酐显著升高(P=0.013和P=0.003)。与对照组相比,肾小球直径的组织病理学检查也较小(P=0.00)。所有数据测量表明与对照组相比存在显著差异。
结论:我们得出结论,低剂量的亚慢性口服毒死蜱可损害肾脏并导致肾衰竭。
BACKGROUND: Chlorpyrifos belongs to a broad-spectrum organophosphate insecticide that has high toxicity, is metabolized in the liver by the oxidation reaction, and can inhibit acetylcholinesterase activity. Acetylcholinesterase inhibition generates the reactive oxygen species and induces oxidative stress, which ultimately results in cellular damage like in the kidney. Examining blood urea nitrogen (BUN) levels, creatinine, and kidney histopathology is an appropriate indicator to assess the toxicity of chlorpyrifos to the degree of damage to cells and kidney tissue.
METHODS: This research used to determine the effect of duration of exposure to
chlorpyrifos and dose-response relationships is important for early detection of the effects of
chlorpyrifos toxicity on health. The research study was a true experimental (completely randomized design) consisting of 30 subjects divided into 5 groups. Controlled Group (K1) given 1 mg/kg BW Tween 20 and NaCl 0, 9% until the 56th day. The
chlorpyrifos exposed group (P1, P2, P3, and P4) was given
chlorpyrifos 5 mg/kg BW for 7, 14, 28, and 56 days. After the treatment, BUN and creatinine levels were measured, and microscopic changes in the kidney were analyzed. The results of BUN, creatinine, and kidney histopathologic were analyzed using the analysis of variance statistical test.
RESULTS: The data result showed that compared to the control group, there were significant increases of BUN and creatinine (P = 0.013 and P = 0.003). Histopathological examinations of kidney glomerulus diameter were also smaller compared to the control group (P = 0.00). All the data measurement indicates significant differences compared to the control group.
CONCLUSIONS: We concluded that sub-chronic oral exposure to
chlorpyrifos at low doses can damage the kidneys and cause kidney failure.