结肠腺癌(COAD)中的钙激活氯通道(CLCA4)和免疫浸润尚未得到广泛研究。这项工作彻底采用了几个数据集来评估表达,预后,以及CLCA4在癌症中的免疫浸润与临床病理特征之间的关系,以及研究潜在的信号通路。人类蛋白质图谱(HPA),TIMER,UALCAN,TISIB,GSCA,SangerBox,遗传狂躁症,和LinkedOmics是使用的数据集之一。研究结果表明,与正常组织相比,COAD组织的CLCA4表达水平较低。CLCA4表达水平较低的患者预后较差。对于验证,使用免疫组织化学(HPA)。CLCA4mRNA表达与其拷贝数变异(CNV)呈正相关,CLCA4CNV与免疫浸润有关。随后的调查证明了免疫细胞标志物之间的关联,免疫检查点基因,和CLCA4的免疫浸润。M0患者的总生存期和无病生存期明显优于M1患者,肿瘤分期M0和M1组的CLCA4表达水平明显不同。其在离子通道活性中的大量富集,跨膜转运蛋白活性,消化,基因本体论分析揭示了其他生物过程。氧化磷酸化,胰腺分泌,帕金森病和阿尔茨海默病,肾素分泌,和其他信号通路是CLCA4的主要关联。很明显,免疫微环境和功能像离子运输,新陈代谢,和肠道消化都受到CLCA4表达的影响。
The calcium-activated chloride channel (CLCA4) in colon adenocarcinoma (COAD) and immunological infiltration have not been extensively studied. This work thoroughly employed several datasets to assess the expression, prognosis, and association between immune infiltration and clinicopathological characteristics of CLCA4 in cancer, as well as look into potential signalling pathways. The human protein atlas (HPA), TIMER, UALCAN, TISIDB, GSCA, SangerBox, GeneMANIA, and LinkedOmics were among the datasets that were used. The findings demonstrated that, in comparison to normal tissues, COAD tissues had lower levels of CLCA4 expression. The prognosis was worse for those whose levels of CLCA4 expression were lower. For validation, immunohistochemistry (HPA) was used. Positive correlations between CLCA4 mRNA expression and its copy number variation (CNV) were observed, and CLCA4 CNV was linked to immunological infiltration. Subsequent investigation demonstrated the association between immune cell markers, immune checkpoint genes, and immunological infiltration with CLCA4. The overall survival and disease-free survival of M0 patients were considerably better than those of M1 patients, and the groups with tumour stages M0 and M1 had notably different levels of CLCA4 expression. Its substantial enrichment in ion channel activity, transmembrane transporter activity, digestion, and other biological processes was revealed by gene ontology analysis. Oxidative phosphorylation, pancreatic secretion, Parkinson\'s and Alzheimer\'s diseases, renin secretion, and other signalling pathways were the primary associations found for CLCA4. It is evident that the immunological microenvironment and functions like ion transport, metabolism, and intestinal digestion are all impacted by CLCA4 expression.