Cluboot病,它是由专性生物营养原生生物油菜疟原虫引起的,导致了疣的形成,通常被称为病原体诱导的肿瘤,在受感染植物的根部。确定宿主肿瘤形成的关键调节因子对于阐明植物细胞内十字花菜菌增殖和分化的潜在机制至关重要。为了深入了解这个过程,进行了转录组学分析,以确定与大白菜中油菜原发和继发感染相关的关键基因。我们的结果表明,子类1的k均值聚类表现出特定的趋势,与油菜的感染过程密切相关。在第1亚类中注释的1610个差异表达基因(DEGs)中,有782个被鉴定为属于49个转录因子家族的转录因子,包括bHLH,B3、NAC、MYB_related,WRKY,bZIP,C2H2和ERF。在原发感染中,几个基因,包括预测的甘蓝型油菜可能的果胶酸裂解酶,RPM1相互作用蛋白4样,含L型凝集素结构域的受体激酶,G型凝集素S受体样丝氨酸,B.rapa光系统II22kDa蛋白,和MLP样蛋白,显示出显着的上调。在继发感染阶段,50个重叠DEG中的45个被上调。这些上调的DEGs包括预测的B.rapa内切葡聚糖酶,长链酰基辅酶A合成酶,WRKY转录因子,含NAC结构域的蛋白质,细胞分裂控制蛋白,生长素诱导蛋白,以及复合触发的根生长反应样和木葡聚糖糖基转移酶中的蛋白质变异。在原发和继发感染阶段,预测DEGs是芸苔属推定的抗病蛋白,含L型凝集素结构域的受体激酶,铁氧还蛋白-NADP还原酶,1-氨基环丙烷-1-羧酸合酶,组蛋白脱乙酰酶,UDP-糖基转移酶,推定的甘油-3-磷酸转运体,和叶绿素a结合蛋白,与植物防御反应密切相关,生物合成过程,碳水化合物运输,和光合作用。这项研究揭示了转录因子在初次感染阶段的感染起始和细胞内寄生关系的建立中的关键作用,以及继发感染阶段宿主细胞内病原体的增殖和分化。
Clubroot disease, which is caused by the obligate biotrophic protist Plasmodiophora brassicae, leads to the formation of galls, commonly known as pathogen-induced tumors, on the roots of infected plants. The identification of crucial regulators of host tumor formation is essential to unravel the mechanisms underlying the proliferation and differentiation of P. brassicae within plant cells. To gain insight into this process, transcriptomic analysis was conducted to identify key genes associated with both primary and secondary infection of P. brassicae in Chinese cabbage. Our results demonstrate that the k-means clustering of subclass 1, which exhibited specific trends, was closely linked to the infection process of P. brassicae. Of the 1610 differentially expressed genes (DEGs) annotated in subclass 1, 782 were identified as transcription factors belonging to 49 transcription factor families, including bHLH, B3, NAC, MYB_related, WRKY, bZIP, C2H2, and ERF. In the primary infection, several genes, including the predicted Brassica rapa probable pectate lyase, RPM1-interacting protein 4-like, L-type lectin-domain-containing receptor kinase, G-type lectin S-receptor-like serine, B. rapa photosystem II 22 kDa protein, and MLP-like protein, showed significant upregulation. In the secondary infection stage, 45 of 50 overlapping DEGs were upregulated. These upregulated DEGs included the predicted B. rapa endoglucanase, long-chain acyl-CoA synthetase, WRKY transcription factor, NAC domain-containing protein, cell division control protein, auxin-induced protein, and protein variation in compound-triggered root growth response-like and xyloglucan glycosyltransferases. In both the primary and secondary infection stages, the DEGs were predicted to be Brassica rapa putative disease resistance proteins, L-type lectin domain-containing receptor kinases, ferredoxin-NADP reductases, 1-aminocyclopropane-1-carboxylate synthases, histone deacetylases, UDP-glycosyltransferases, putative glycerol-3-phosphate transporters, and chlorophyll a-binding proteins, which are closely associated with plant defense responses, biosynthetic processes, carbohydrate transport, and photosynthesis. This study revealed the pivotal role of transcription factors in the initiation of infection and establishment of intracellular parasitic relationships during the primary infection stage, as well as the proliferation and differentiation of the pathogen within the host cell during the secondary infection stage.