目的:本研究的目的是阐明中国汉族人群中HLAⅠ类和Ⅱ类等位基因与强直性脊柱炎(AS)的相关性。
方法:我们对68个HLA-B*27(-),62例HLA-B*27(+)AS患者,70个控件。对HLA-B*27(-)患者进行病例对照分析和单独分析。采用单因素方差分析和Kruskal-Wallis多重比较检验分析HLA-A\\B\\C\\DRB1\\DQB1等位基因对HLA-B*27(-)和HLA-B*27(+)患者临床特征的影响。
结果:在HLA-B*27(+)组中,A*11:02,B*27:04,B*27:05,C*02:02,C*12:02和DRB1*04:01为正相关,A*33:03,B*07:02,B*57:01和C*07:02为负相关.HLA-B*27(-)患者的发病年龄大于HLA-B*27()患者(30.03±15.15vs.23.08±7.79年)。在HLA-B*27(-)组中,具有A*01:01,B*13:01,B*13:02,C*01:02,C*04:01,DQB1*02:01,DQB1*06:01和DRB1*03:01的发病时间比没有这些等位基因的早,而携带B*40:02,C*07:02,C*12:02,C*15:02,DQB1*05:02和DQB1*05:03的患者有延迟发作。在HLA-B*27(-)组中,A*32:01(+),C*08:01(+),和DRB1*04:05(-)女性可能发展为AS。在HLA-B*27(+)组中,DQB1*03:02(+)女性可能更容易患AS。DRB1*12:02和HLA-B*27与AS感染部位的分布相互作用。在HLA-B*27(+)组中,DRB1*12:02(+)患者可能有外周关节受累。
结论:HLA-B*27以外的HLAI类和II类等位基因与中国汉族患者的AS易感性和特征有关。
OBJECTIVE: The objective of the study was to clarify the associations of HLA class I and II alleles with ankylosing spondylitis (AS) among Chinese Han.
METHODS: We performed HLA genotyping and Sanger sequencing for 68 HLA-B*27(-), 62 HLA-B*27(+) AS patients, and 70 controls. Case-control analyses and separate analyses of HLA-B*27(-) patients were performed. One-way ANOVA and Kruskal-Wallis multiple comparisons test were used to analyze the effects of HLA-A\\B\\C\\DRB1\\DQB1 alleles on clinical characteristics of HLA-B*27(-) and HLA-B*27(+) patients.
RESULTS: In the HLA-B*27(+) group, positive associations were seen with A*11:02, B*27:04, B*27:05, C*02:02, C*12:02, and DRB1*04:01 and negative associations were seen with A*33:03, B*07:02, B*57:01, and C*07:02. The age at onset was greater in HLA-B*27(-) patients than in HLA-B*27(+) patients (30.03 ± 15.15 vs. 23.08 ± 7.79 years). In the HLA-B*27(-) group, those with A*01:01, B*13:01, B*13:02, C*01:02, C*04:01, DQB1*02:01, DQB1*06:01, and DRB1*03:01 had an earlier onset than those without these alleles, while patients carrying B*40:02, C*07:02, C*12:02, C*15:02, DQB1*05:02, and DQB1*05:03 had a delayed onset. In the HLA-B*27(-) group, A*32:01(+), C*08:01(+), and DRB1*04:05(-) women were likely to develop AS. In the HLA-B*27(+) group, DQB1*03:02(+) women may be more likely to develop AS. DRB1*12:02 and HLA-B*27 interacted with the distribution of AS-affected sites. In the HLA-B*27(+) group, DRB1*12:02(+) patients were likely to have peripheral joint involvement.
CONCLUSIONS: HLA class I and II alleles other than HLA-B*27 contribute to AS predisposition and characteristics among Chinese Han patients.