随着乳腺癌成为一个紧迫的全球健康挑战,其特点是发病率和地理差异不断上升,迫切需要创新的治疗策略。这项全面的研究导航纳米医学的景观,特别关注磁性纳米粒子(MNPs)的潜力,以磁铁矿(Fe3O4)为中心。MNPs,封装在生物相容性聚合物如二氧化硅被称为磁性二氧化硅纳米颗粒(MSN),用磷钨酸盐(PTA)增强化学动力学疗法(CDT)。PTA以其作为天然螯合剂和电子穿梭的双重作用而闻名,加快纳米颗粒内的三价铁(Fe3+)到亚铁(Fe2+)离子的电子转移。此外,引入基于蛋白质的电荷逆转纳米载体,如丝丝胶和麸质,以封装(MSN-PTA)纳米颗粒,为药物输送系统提供了一个动态的方面,为乳腺癌治疗的潜在变革提供了一个动态的方面。这项研究成功地制定和表征蛋白质包被的纳米胶囊,特别是MSN-PTA-SER,和MSN-PTA-GLU,具有药物输送应用的最佳物理化学属性。丝胶和面筋浓度的仔细优化导致精细调整的纳米粒子,展示均匀尺寸,负zeta电位增强,和显著的稳定性。各种分析,从动态光散射(DLS)和扫描电子显微镜(SEM)到透射电子显微镜(TEM),傅里叶变换红外光谱(FTIR),X射线衍射分析(XRD),和热重分析(TGA),提供对结构完整性和表面改性的见解。振动样品磁强计(VSM)分析强调超顺磁行为,将这些纳米胶囊定位为靶向药物递送的有希望的候选药物。体外评估显示MCF-7和Zr-75-1乳腺癌细胞中细胞活力的剂量依赖性抑制,强调MSN-PTA-SER和MSN-PTA-GLU的治疗潜力。表面电荷和pH依赖性细胞摄取的相互作用突出了这些纳米载体在肿瘤微环境中的强大稳定性和多功能性。为靶向药物递送和个性化纳米医学的进步铺平了道路。这项比较分析探讨了丝胶和面筋的适用性,为先进的发展开辟了一条有希望的道路,有针对性的,和有效的乳腺癌治疗。
With breast cancer emerging as a pressing global health challenge, characterized by escalating incidence rates and geographical disparities, there is a critical need for innovative therapeutic strategies. This comprehensive research navigates the landscape of nanomedicine, specifically focusing on the potential of magnetic nanoparticles (MNPs), with magnetite (Fe3O4) taking center stage. MNPs, encapsulated in biocompatible polymers like silica known as magnetic silica nanoparticles (MSN), are augmented with phosphotungstate (PTA) for enhanced chemodynamic therapy (CDT). PTA is recognized for its dual role as a natural chelator and electron shuttle, expediting electron transfer from ferric (Fe3+) to ferrous (Fe2+) ions within nanoparticles. Additionally, protein-based charge-reversal nanocarriers like silk sericin and gluten are introduced to encapsulate (MSN-PTA) nanoparticles, offering a dynamic facet to drug delivery systems for potential revolutionization of breast cancer therapy. This study successfully formulates and characterizes protein-coated nanocapsules, specifically MSN-PTA-SER, and MSN-PTA-GLU, with optimal physicochemical attributes for drug delivery applications. The careful optimization of sericin and gluten concentrations results in finely tuned nanoparticles, showcasing uniform size, enhanced negative zeta potential, and remarkable stability. Various analyses, from Dynamic Light Scattering (DLS) and scanning electron microscopy (SEM) to transmission electron microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR), X-Ray diffraction analysis (XRD), and Thermogravimetric analysis (TGA), provide insights into structural integrity and surface modifications. Vibrating Sample Magnetometer (VSM) analysis underscores superparamagnetic behavior, positioning these nanocapsules as promising candidates for targeted drug delivery. In vitro evaluations demonstrate dose-dependent inhibition of cell viability in MCF-7 and Zr-75-1 breast cancer cells, emphasizing the therapeutic potential of MSN-PTA-SER and MSN-PTA-GLU. The interplay of surface charge and pH-dependent cellular uptake highlights the robust stability and versatility of these nanocarriers in tumor microenvironment, paving the way for advancements in targeted drug delivery and personalized nanomedicine. This comparative analysis explores the suitability of silk sericin and gluten, unraveling a promising avenue for the development of advanced, targeted, and efficient breast cancer treatments.