开发纳米粒子(NPs)的简单绿色方法引起了研究人员的关注。关于利用叶提取物制备氧化铈(CeO2NP)的文献很少。本研究合成了叶介导的CeO2NP,以生产尺寸可控的纳米粉体,以供进一步应用。该研究是第一个报告优化参数(pH7,5g/150mL浓度的叶提取物,和3小时的反应时间)使用Mastimomasp.获取CeO2NP。叶提取物作为盖帽剂具有优异的性能。在该研究中获得的NP悬浮液的吸光度用紫外-可见(UV-Vis)光谱法在约252nm处记录。傅里叶变换红外光谱(FTIR),扫描电子显微镜(SEM),X射线衍射(XRD)和透射电子显微镜(TEM)也用于表征和确认制备的CeO2NP。XRD光谱记录了特定衍射图案下NP的纯度,而TEM揭示了具有16nm粒径的纳米颗粒的球形形式。从获得的FTIR光谱中已经证实了CeO2NP的形成,其在555nm处表现出Ce-O峰。植物化学筛选试验和叶提取物的FT-IR分析揭示了黄酮类化合物的存在,萜类化合物,糖,糖皂苷,醌,和糖苷。不同浓度的NP悬浮液(对照,制备50、100、150、200和250μg/mL)并用于针对革兰氏阳性和阴性细菌的评估。结果,CeO2NP对两种细菌均具有抗菌活性。在1.83±0.137和1.83±0.14mm的最大抑制区,对大肠杆菌和肺炎克雷伯菌的抗菌活性最高。分别,在250mg/uL的NP。
Simple and green methods of developing nanoparticles (NPs) have attracted the attention of researchers. Literature on utilising leaf extract to prepare cerium oxide (CeO2 NPs) is scarce. The present study synthesised leaf-mediated-CeO2 NPs to produce nanopowders of controllable sizes for further applications. The study is the first to report the optimised parameters (pH 7, 5 g/150 mL concentration of the leaf extract, and 3 h of reaction time) of procuring CeO2 NPs using Melastoma sp. leaf extract as the capping agent with excellent properties. The absorbance of the NPs suspension obtained in this study was recorded at approximately 252 nm with Ultraviolet-Visible (UV-Vis) Spectroscopy. Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), and Transmission Electron Microscopy (TEM) were also utilised to characterise and confirm the CeO2 NPs prepared. The XRD spectra documented the purity of the NPs at specific diffraction patterns, while TEM revealed the spherical form of the NPs with a particle size of 16 nm. The formation of CeO2 NPs has been confirmed from the FTIR spectra procured, which exhibited a Ce-O peak at 555 nm. Phytochemical screening test and FT-IR analysis of leaf extract revealed the existence of flavonoids, terpenoids, sugars, saponins, quinones, and glycosides. The NPs suspensions of varying concentrations (control, 50, 100, 150, 200, and 250 μg/mL) were prepared and employed for evaluations against Gram-positive and -negative bacteria. Resultantly, CeO2 NPs demonstrated antibacterial activities against both bacteria types. The highest antibacterial activities were recorded against E. coli and K. pneumonia at 1.83 ± 0.137 and 1.83 ± 0.14 mm maximum inhibition zones, respectively, at 250 mg/uL of the NPs.