目的:本研究的目的是评估脑活素(CBL)在局部照射后对大鼠脑组织的辐射防护作用。
背景:CBL已经证明了抗氧化剂,抗炎,和组织修复特性。在这项研究中,评价CBL对大鼠脑组织的辐射防护作用(50mg/kg)。
目的:不同氧化应激标志物的水平,包括丙二醛(MDA),一氧化氮(NO),谷胱甘肽过氧化物酶(GPx),过氧化氢酶(CAT),用辐射和CBL处理后检查超氧化物歧化酶(SOD)。
方法:首先,使用20只体重180-200g的雄性成年Wistar大鼠。使用线性加速器单元以200cGy/mine的剂量率将动物暴露于15Gy的单个部分。在这项研究中,为了检查IR后的氧化应激量,四种标志物MDA的水平,NO,GPx,CAT,使用分光光度法和购买的试剂盒检查和测量SOD。
结果:结果表明,与IR组相比,CBL的给药显著增加GPX和SOD的水平(p<0.05)。
结论:我们的发现表明,CBL通过增强抗氧化防御机制对大脑具有辐射防护作用。
OBJECTIVE: The aim of this study is to evaluate radioprotective effects of
Cerebrolysin (CBL) in rats\' brain tissues after local irradiation.
BACKGROUND: CBL has demonstrated antioxidant, anti-inflammatory, and tissue repair properties. In this study, the radioprotective effects of CBL in the brain tissues of rats after Irradiation (IR) (50 mg/ kg) were evaluated.
OBJECTIVE: The levels of different oxidative stress markers, including malondialdehyde (MDA), nitric oxide (NO), glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) were examined after treatment with radiation and CBL.
METHODS: First, 20 male adult Wistar rats weighing 180-200 g were used. The animals were exposed to a single fraction of 15Gy using a linear accelerator unit at a dose rate of 200 cGy/mine. In this study, to check the amount of oxidative stress following the IR, the level of four markers MDA, NO, GPx, CAT, and SOD were examined and measured using the spectrophotometric method and purchased kits.
RESULTS: The results showed that compared to the IR group, the administration of CBL increases the levels of GPX and SOD significantly (p < 0.05).
CONCLUSIONS: Our finding suggests that CBL has radioprotective effects on the brain by enhancing antioxidant defense mechanisms.