四水平黄芪多糖(AP)日粮补充的效果,(CD:对照饮食和三个实验饮食(E),EA:100mgkg-1AP;EB:200mgkg-1AP;EC:300mgkg-1AP)对生长,血液生化参数和代谢消化酶的变化,增强抗氧化活性,先天-适应性免疫反应,在Catla(Catlacatla)中研究了细胞因子基因表达。饲喂CD的健康组和挑战组没有显示死亡率,而喂食EA或EC的鱼显示10%的死亡率,但在饮食EB中死亡率仅为5%。在实验期间,饲喂饮食EB和EC的鱼显示出明显更好的生长速率和较高的RBC计数。当从第6-8周开始给鱼饲喂饮食EB和EC时,白蛋白和球蛋白水平显着提高。从第4-8周开始,EB饲喂可显著改善超氧化物歧化酶(SOD)。相比之下,血清髓过氧化物酶(MPO),过氧化氢酶(CAT),丙二醛(MDA)/脂质过氧化(LPO),谷胱甘肽过氧化物酶(GPx),呼吸爆发活动(RBA),杀菌作用(BCA),血清溶菌酶活性(SLA),一氧化氮合酶(NOS),头肾白细胞反应增殖(HKLP),溶血作用(HLA),过氧化氢(H2O2),免疫球蛋白(Ig)从第6-8周明显改善。饲喂富含AP饮食的组在饲喂6周和8周后,白细胞介素(IL)-1β和干扰素(IFN)-γmRNA的表达显着改善。然而,IL-10和主要组织相容性复合物(MHC)-1mRNA表达在第8周饲喂所有AP饮食的Catla中均显着增强。富含AP的饮食显示,第4周时肿瘤坏死因子(TNF)-α和TNF受体相关因子6(TRAF6)mRNA表达显着改善,但Toll样受体2(TLR2)和TLR4mRNA表达显着增强饮食EB和EC在第6周和第8周之后。同样,头肾(HK)中的溶菌酶(Lyz)-C和Lyz-GmRNA水平通过在第6周和第8周喂食AP而增加,而EB饮食,核苷酸结合寡聚化结构域-1(NOD1)的表达在第6周和第8周显著改善,但NOD2mRNA的表达仅在8周后显著增强.通过喂养健康的Catla和E.tarda挑战鱼类喂养饮食EB,导致显着增加的增长,血液生化指标,代谢消化酶,抗氧化活性,先天-适应性免疫反应,细胞因子基因表达主要在6~8周。
The effect of four level of Astragalus polysaccharides (APs) supplementation diets, (CD: control diet and three experiment diet (E), EA: 100 mg kg-1 APs; EB: 200 mg kg-1 APs; EC: 300 mg kg-1 APs) on growth, changes in haemato-biochemical parameters and metabolic-digestive enzymes, enhancement of antioxidant activity, innate-adaptive immune response, and cytokine gene expression were studied in catla (Catla catla) against Edwardsiella tarda. The healthy and challenged groups fed the CD displayed no mortality, while fish fed EA or EC revealed 10% mortality, but the mortality was only 5% in diet EB. Fish fed diet EB and EC revealed significantly better growth rates and high RBC count during the experimental period. Albumin and globulin levels were significant improved when fish were fed the diet EB and EC from weeks 6-8. The superoxide dismutase (SOD) was significant ameliorated by EB feeding from weeks 4-8. In contrast, serum myeloperoxidase (MPO), catalase (CAT), malondialdehyde (MDA)/lipid peroxidation (LPO), glutathione peroxidase (GPx), respiratory burst activity (RBA), bactericidal action (BCA), serum lysozyme activity (SLA), nitric oxide synthase (NOS), head kidney leukocytes response proliferation (HKLP), hemolytic action (HLA), hydrogen peroxides (H2O2), and immunoglobulin (Ig) were significantly improved from week 6-8. Groups fed the APs enriched diets had significant ameliorated interleukin (IL)-1β and interferon (IFN)-γ mRNA expression after 6 and 8 weeks of feeding. However, IL-10 and major histocompatibility complex (MHC)-1 mRNA expressions were significant enhanced in catla fed all APs diets on week 8. APs enriched diets revealed significant improved tumor necrosis factor (TNF)-α and TNF receptor-associated factor-6 (TRAF6) mRNA expression on week 4, but toll-like receptor-2 (TLR2) and TLR4 mRNA expression were significant enhanced by diet EB and EC after weeks 6 and 8. Similarly, the lysozyme (Lyz)-C and Lyz-G mRNA levels in the head kidney (HK) increased by APs feeding on weeks 6 and 8, whereas the EB diet, the expression of nucleotide binding oligomerization domain-1 (NOD1) was significantly improved on weeks 6 and 8, but NOD2 mRNA expression was only significant enhanced after 8 weeks of diet EB. By feeding healthy catla and E. tarda challenged fish fed diet EB, resulted in significantly increased growth, haemato-biochemical indices, metabolic-digestive enzymes, antioxidant activities, innate-adaptive immune responses, and cytokine gene expression mainly between 6 and 8 weeks.