背景:氟哌啶醇(HAL)是一种用于治疗精神分裂症的抗精神病药。然而,由于其系统作用,在锥体外束中观察到不良反应。天然化合物是巴西生物多样性中广泛可用的治疗替代品之一。Mygalin(MY),一种多胺,是由一种天然分子合成的,可能是一种有趣的方法。
目的:本研究旨在评估MY对HAL诱导的僵直症小鼠的影响。
方法:使用雄性瑞士小鼠。通过腹膜内施用在生理盐水中稀释的HAL(0.5mg/kg-1mL/Kg)诱导僵直。为了评估MY对僵直症的影响,将小鼠分为4组:(1)生理盐水(0.9%NaCl);(2)0.002mg/Kg的MY;(3)0.02mg/Kg的MY;(4)0.2mg/Kg的MY。MY或盐水在盐水之前腹膜内(IP)给予10分钟bHAL。在IP施用HAL后15、30、60、90和120分钟使用棒试验评估僵直。
结果:与对照组相比,IP给药HAL后,棒试验15、30、60和90分钟的潜伏期时间增加(p<0.05)。在IP给药0.2mg/Kg的MY后15、30、90和120分钟(p<0.05),而0.02mg/Kg的MY在HAL治疗后15分钟减轻了僵直。我们的发现表明,MY减轻了HAL诱导的小鼠的僵化状态。
BACKGROUND: Haloperidol (HAL) is an antipsychotic used in the treatment of schizophrenia. However, adverse effects are observed in the extrapyramidal tracts due to its systemic action. Natural compounds are among the treatment alternatives widely available in Brazilian biodiversity. Mygalin (MY), a polyamine that was synthesized from a natural molecule present in the hemolymph of the Acanthoscurria gomesian spider, may present an interesting approach.
OBJECTIVE: This study aimed to evaluate the effect of MY in mice subjected to HAL-induced
catalepsy.
METHODS: Male Swiss mice were used. Catalepsy was induced by intraperitoneal administration of HAL (0.5 mg/kg - 1 mL/Kg) diluted in physiological saline. To assess the MY effects on
catalepsy, mice were assigned to 4 groups: (1) physiological saline (NaCl 0.9 %); (2) MY at 0.002 mg/Kg; (3) MY at 0.02 mg/Kg; (4) MY at 0.2 mg/Kg. MY or saline was administered intraperitoneally (IP) 10 min b HAL before saline.
Catalepsy was evaluated using the bar test at 15, 30, 60, 90, and 120 min after the IP administration of HAL.
RESULTS: The latency time in the bar test 15, 30, 60, and 90 min increased (p < 0.05) after IP administration of HAL compared to the control group.
Catalepsy was attenuated 15, 30, 90, and 120 min (p < 0.05) after the IP-administration of MY at 0.2 mg/Kg; while MY at 0.02 mg/Kg attenuated
catalepsy 15 min after the HAL treatment. Our findings showed that MY attenuates the HAL-induced cataleptic state in mice.