蜂蜜的植物来源对蜂蜜的质量控制和商业化很重要。在这项研究中,建立了一种纳升电喷雾质谱(Nano-ESI-MS)方法,Eurya蜂蜜(EH),Dendropanaxdentgerhoney(DH),和三分法蜂蜜(TH)。总的来说,基于通过Nano-ESI-MS的碰撞诱导解离实验鉴定了38种化合物,其中16种差异化合物和7种作为潜在差异标记物定量。这四种蜂蜜通过质谱数据与7种差异标记中的3种进行多变量分析,相互区分。即,苯乙胺,三香豆醇亚精胺,和(+/-)-脱落酸,被确定为CH的潜在标志物,EH,DH,分别。UPLC-Q/TOF-MS进一步验证了Nano-ESI-MS的定性和定量结果。我们的研究为Nano-ESI-MS方法在鉴定不同蜂蜜的植物来源方面提供了重要的潜力。
The botanical origins of honey are important for the quality control and commercialization of honey. In this research, we established a nanoliter electrospray ionization mass spectrometry (Nano-ESI-MS) method to identify
Castanopsis honey (CH), Eurya honey (EH), Dendropanax dentiger honey (DH), and Triadica cochinchinensis honey (TH). In total, 38 compounds were identified based on the collision-induced dissociation experiments by Nano-ESI-MS with 16 differential compounds and 7 quantified as potential differential markers. These four types of honey were distinguished from each other by their mass spectrometry data combined with multivariate analysis with three out of the 7 differential markers, i.e., phenethylamine, tricoumaroyl spermidine, and (+/-)-abscisic acid, identified as potential markers for CH, EH, and DH, respectively. Both the qualitative and quantitative results derived from Nano-ESI-MS were further verified by UPLC-Q/TOF-MS. Our studies provided the significant potential of the Nano-ESI-MS method in the identification of the botanical origins of different kinds of honey.