临床前试验是药物发现的重要基础,发展,和安全过程。在这个过程中,进行动物试验以确定新化合物的安全性和任何潜在的不良反应。进行发育毒性测试以验证药物是否有可能导致发育中的胚胎/胎儿先天性异常。鸡胚对于这些目的非常有用,并且具有几个优点,例如生产和住房的低成本,易于处理和操纵,除了在分子上与人类胚胎有相似之处之外,细胞,和解剖学水平。在这一章中,我们介绍了使用鸡胚模型来测试药物的致畸作用并评估其主要结局的方法。
Pre-clinical trials are an essential step that underpins the drug discovery, development, and safety process. During this process, animal testing is performed to determine the safety of new compounds and any potential adverse effects. Developmental toxicity tests are carried out to verify whether the drug has potential to cause congenital anomalies to the developing embryo/fetus. Chicken embryos are very useful for these purposes and present several advantages, such as low cost of production and housing, easy handling and manipulation, and rapid development in addition to sharing similarities to the human embryo at molecular, cellular, and anatomical levels. In this chapter, we bring methods for using the chicken embryo model for testing the teratogenic effects of drugs and assessing the main outcomes of them.