在这项研究中,我们研究了严重急性呼吸综合征冠状病毒-2(SARS-CoV-2)刺突蛋白是否可以改变血浆中血管紧张素转换酶2(ACE2)的活性,心,肾,肝脏,肺,和六个大脑区域(杏仁核,脑干,皮质,海马体,下丘脑,和纹状体)糖尿病和高血压大鼠。我们测定血浆和心脏裂解物中的ACE2活性,肾,肝脏,肺,和六个大脑区域。MLN-4760抑制血浆和所有器官中的ACE2活性。另一方面,糖尿病大鼠血浆可溶性ACE2(sACE2)活性升高,高血压大鼠的血浆没有变化。ACE2活性在肝脏中增强,脑干,和纹状体,虽然它在肾脏中减少,杏仁核,皮质,和糖尿病大鼠的海马。肾脏ACE2活性增加,肝脏,和肺,虽然它在心脏中减少,杏仁核,皮质,高血压大鼠的下丘脑。我们通过酶联免疫吸附试验测量ACE2含量,发现心脏中ACE2蛋白水平升高,虽然它在血浆中减少,肾,脑干,皮质,海马体,下丘脑,和糖尿病大鼠的纹状体。ACE2蛋白水平在脑干降低,皮质,海马体,高血压大鼠的下丘脑。我们的数据表明,刺突蛋白增强了糖尿病大鼠肝脏和肺部的ACE2活性,以及心脏和三个大脑区域(皮层,下丘脑,和纹状体)高血压大鼠。
In this study, we investigated whether severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein may modify angiotensin-converting enzyme 2 (ACE2) activity in the plasma, heart, kidney, liver, lung, and six brain regions (amygdala, brain stem, cortex, hippocampus, hypothalamus, and striatum) of diabetic and hypertensive rats. We determine ACE2 activity in the plasma and lysates of heart, kidney, liver, lung, and six brain regions. MLN-4760 inhibits ACE2 activity in the plasma and all organs. On the other hand, soluble ACE2 (sACE2) activity increased in the plasma of diabetic rats, and there was no change in the plasma of hypertensive rats. ACE2 activity was augmented in the liver, brain stem, and striatum, while it decreased in the kidney, amygdala, cortex, and hippocampus of diabetic rats. ACE2 activity increased in the kidney, liver, and lung, while it decreased in the heart, amygdala, cortex, and hypothalamus of hypertensive rats. We measured the ACE2 content via enzyme-linked immunosorbent assay and found that ACE2 protein levels increased in the heart, while it decreased in the plasma, kidney, brain stem, cortex, hippocampus, hypothalamus, and striatum of diabetic rats. ACE2 protein levels decreased in the brain stem, cortex, hippocampus, and hypothalamus of hypertensive rats. Our data showed that the spike protein enhanced ACE2 activity in the liver and lungs of diabetic rats, as well as in the heart and three of the brain regions (cortex, hypothalamus, and striatum) of hypertensive rats.